CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S386
DOI: 10.1055/s-0038-1641053
Poster
Tissue Engineering/Stammzellen: Tissue Engineering/Stem Cells

Influence of electrical stimulation on survival and growth of spiral ganglion neurons in vitro

M Peter
1   MHH/Klinik für Hals-, Nasen-, Ohrenheilkunde, Hannover
,
U Reich
2   Klink für Hals-, Nasen- und Ohrenheilkunde, Charité, Berlin
,
A Warnecke
3   Klink für Hals-, Nasen- und Ohrenheilkunde, Medizinische Hochschule Hannover, Hannover
,
H Olze
2   Klink für Hals-, Nasen- und Ohrenheilkunde, Charité, Berlin
,
A Szczepek
2   Klink für Hals-, Nasen- und Ohrenheilkunde, Charité, Berlin
,
T Lenarz
3   Klink für Hals-, Nasen- und Ohrenheilkunde, Medizinische Hochschule Hannover, Hannover
,
G Paasche
3   Klink für Hals-, Nasen- und Ohrenheilkunde, Medizinische Hochschule Hannover, Hannover
› Author Affiliations
Gefördert durch die Deutsche Forschungsgesellschaft (WA2806/5-1)
 

Introduction:

More and more patients with residual hearing (RH) are candidates for cochlear implantation (CI). However, some of them experiences post-implantation late hearing loss of which the etiology has not been not well understood. The surgical trauma occurring during implantation is unlikely the reason for the observed hearing loss. Evidence exists about electrical stimulation (ES) being possibly responsible for the adverse effect on spiral ganglion neurons (SGN). Therefore, the aim of this study was to examine survival and neurite growth of SGN in vitro under ES.

Methods:

Stimulation setup was designed to provide defined electrical fields at given points of the stimulation chamber. Dissociated SGN from rats (p3 – 4) were cultured for 24h and then exposed to biphasic, pulsed electrical stimulation (interpulse delay 120µs, repetition rate 1 kHz) for another 24h. The current has varied in the range of 0 to 2 mA. The pulse width ranged from 10 to 400µs at a current of 1 mA. After stimulation, the cultures were washed, fixed and stained to detect the neurofilament of SGN. The neurite growth and survival of SGN were evaluated with respect to the electrical field at the position of the cells. Non-stimulated cultures were included as controls.

Results:

The current of 2 mA has induced total loss of SGN. The survival of SGN was reduced at 1 mA and depended on the electrical field at the position of the individual cells. Overall, survival and neurite growth of SGN were inversely proportional to the applied electrical field.

Conclusions:

In the first experiments, the developed electrical stimulation setup proved to be a good model for the in vitro investigation of electro-biological effects in the inner ear and provided evidence for SGN electro-toxicity in over stimulation.



Publication History

Publication Date:
18 April 2018 (online)

© 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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