CC BY-NC-ND 4.0 · Laryngorhinootologie 2018; 97(S 02): S386-S387
DOI: 10.1055/s-0038-1641055
Poster
Tissue Engineering/Stammzellen: Tissue Engineering/Stem Cells

Regenerative Medicine of the vocal fold: Magnetic Tissue Engineering (MTE) using superparamagnetic ironoxide nanoparticles

M Pöttler
1   Universitätsklinikum Erlangen/HNO-Klinik/SEON, Erlangen
,
E Schreiber
2   Hals-Nasen-Ohren-Klink, SEON – Sektion für Experimentelle Onkologie und Nanomedi, Erlangen
,
S Dürr
3   Hals-Nasen-Ohren-Klink, Abteilung für Phoniatrie und Pädaudiologie, Universitäts, Erlangen
,
M Döllinger
3   Hals-Nasen-Ohren-Klink, Abteilung für Phoniatrie und Pädaudiologie, Universitäts, Erlangen
,
C Alexiou
2   Hals-Nasen-Ohren-Klink, SEON – Sektion für Experimentelle Onkologie und Nanomedi, Erlangen
› Author Affiliations
 

Introduction:

The voice is the most important instrument of communication. Losing one's ability to speak due to tissue loss in this area leads to serious impairment in the quality of life. Conventional methods for tissue engineering of the VF are so fare ineffective. Thus, techniques to manipulate and remotely control cells can deliver a powerful tool for regenerative medicine. But, in order to function properly, the complex interplay between epithelial cells and fibroblasts, which is necessary to build the intricate structure of the VF, has to be established.

Methods:

The innovative approach of Magnetic Tissue Engineering (MTE) provides a fast and precise method of generating functional multi-layered cell-constructs. The underlying concept includes cellular uptake of superparamagnetic iron oxide nanoparticles (SPIONs) and the usage of external magnetic fields to manipulate and remotely control magnetized cells and their behaviour.

Results:

After we established the best ratio in both, epithelial cells and fibroblasts, between SPION uptake, good cell-magnetisation and tolerability we were able to generate multi-layered 3D VF cell- constructs. Transplantation into rabbit larynxes, imaging as well as functional and structural analysis give further insights about the successful realisation and efficiency of this technique.

Conclusion:

Our results will constitute a solid basis for a successful transfer of this technique into humans, in order to provide a functional and personalized vocal fold transplant. This is particularly important for patients, who suffer from dysphonia as a consequence of a vocal fold tissue defect, and will help to improve their quality of life.

Acknowledgments:

Deutsche Krebshilfe Nr. 111332



Publication History

Publication Date:
18 April 2018 (online)

© 2018. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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