Radioimmunoassay of an Early Plasmin Degradation Product of Human Fibrinogen, “Fragment A”, and its Clinical Application

Kimiteru Takagi; Tadashi Kawai

doi:10.1055/s-0038-1646118

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1978; 39(01): 001-011
DOI: 10.1055/s-0038-1646118

Original Article

Schattauer GmbH Stuttgart

Radioimmunoassay of an Early Plasmin Degradation Product of Human Fibrinogen, “Fragment A”, and its Clinical Application

Authors

Kimiteru Takagi

The Department of Clinical Pathology, Jichi Medical School, 3311—1, Yakushiji, Minamikawachmachi, Kawachigun, Tochigiken, Japan
Tadashi Kawai

The Department of Clinical Pathology, Jichi Medical School, 3311—1, Yakushiji, Minamikawachmachi, Kawachigun, Tochigiken, Japan

Abstract

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Summary

Upon the plasmin digestion of human fibrinogen, an early cleavage product, which has been designated as fragment A, was isolated, and to study the action of plasmin in the circulation, radioimmunoassay for fragment A was carried out. This assay used rabbit immune serum obtained by injection of fragment A mixed with complete Freund’s adjuvant, and fragment A was labeled with ¹²⁵I using the Chloramin-T method.

In 20 normal healthy donors its serum level was 3.57 ± 1.62 μg/ml (mean ± SD), and it was increased significantly in certain diseases, such as acute leukemias, cardiovascular disorders, malignancies, renal failure, systemic lupus erythematosus and sepsis.

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