Catabolism of Fibrinogen in Pregnant Rabbits before and after Delivery

Ingo Mahn; Richard-Mathias Moeller; Gert Müller-Berghaus

doi:10.1055/s-0038-1646651

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1978; 39(01): 032-038
DOI: 10.1055/s-0038-1646651

Original Article

Schattauer GmbH Stuttgart

Catabolism of Fibrinogen in Pregnant Rabbits before and after Delivery

Authors

Ingo Mahn

Division of Clinical Immunology and Blood Transfusion, Central Division of the Department of Nuclear Research, and the Department of Medicine, Justus-Liebig-Universität, Giessen, West-Germany
Richard-Mathias Moeller

Division of Clinical Immunology and Blood Transfusion, Central Division of the Department of Nuclear Research, and the Department of Medicine, Justus-Liebig-Universität, Giessen, West-Germany
Gert Müller-Berghaus

Division of Clinical Immunology and Blood Transfusion, Central Division of the Department of Nuclear Research, and the Department of Medicine, Justus-Liebig-Universität, Giessen, West-Germany

Abstract

PDF Download

Summary

16 nón-pregnant female and 19 pregnant rabbits were injected with purified rabbit ¹²⁵I-fibrinogen in order to study the catabolism of fibrinogen before and after delivery. The half-life time (T ½) of the clottable ¹²⁵I-radioactivity in pregnant rabbits during the last third of gestation was 27.3 ± 5.3 hr in comparison to 54.4 ± 3.7 hr in non-pregnant rabbits. After delivery, T ½ returned to normal values of 47.9 ± 10.9 hr. The fractional catabolic rate (FCR) of the clottable ¹²⁵I-radioactivity was 67.1% × d⁻¹ ± 8.6 before and 41.6% × d⁻¹ after delivery whereas FCR in non-pregnant rabbits amounted to 44.7% × d⁻¹ ± 4.8. These figures demonstrate a pronounced increase in fibrinogen catabolism during the last third of gestation in pregnant rabbits and a normalization immediately after delivery. Although in pregnant rabbits the elimination of fibrinogen from the circulating blood was pronouncedly increased, the plasma fibrinogen concentration did not change. Only after delivery did the fibrinogen concentration increase when the fibrinogen catabolism had already normalized.

PDF (675 kb)

References

References
1 Beller FK, Douglas GW, Morris RH, Johnson AJ. 1968; The fibrinolytic enzyme system in pregnancy. A comparison between ovarian and antecubital blood. American Journal of Obstetrics and Gynecology 101: 587
2 Brakman P. 1966; The fibrinolytic system in human blood during pregnancy. American Journal of Obstetrics and Gynecology 94: 14
3 Chandra RK, Malkani PK, Bhasin K. 1973; Serum levels of α₂-macroglobulin and IgG and cryofibrinogenemia in pregnancy. Obstetrics and Gynecology 41: 56
4 Dost FH. 1953. Der Blutspiegel. Kinetik der Konzentrationsabläufe in der Kreislaüffliissigkeit. Georg Thieme Verlag; Leipzig:
5 Glueck HI, Herrmann LG. 1964; Cold-precipitable fibrinogen, “cryofibrinogen”. Archives of Internal Medicine 113: 748
6 Graeff H, von Hugo R, Hafter R. 1973; In vivo formation of soluble fibrin monomer complexes in human plasma. Thrombosis Research 3: 465
7 Hafter R, schneebauer T, Tafel K, Ernst E, Graeff H. 1975; Bestimmung von löslichen Fi-brinmonomerkomplexen zur Erfassung der Hyperkoagulabilität in der Schwangerschaft und unter der Geburt. Geburtshilfe und Frauenheilkunde 35: 518
8 Hahn L. 1974; On fibrinolysis and coagulation during parturition and menstruation. Acta Obstetrica et Gynecologica Scandinavica Suppl (Suppl. 28) 1
9 Jacobsson K. 1955; Studies on fibrinogen. I. Studies on the determination of fibrinogen in human blood plasma. Scandinavian Journal of Clinical and Laboratory Investigation 7 Suppl (Suppl. 14) 1
10 Kitzmiller JL, Lang JE, Yelenosky PF, Lucas WE. 1974; Hematologic assays in pre-eclampsia. American Journal of Obstetrics and Gynecology 118: 362
11 Mahn I, Müller-Berghaus G. 1975; Studies on catabolism of ¹²⁵I-labelled fibrinogen in normal rabbits and in rabbits with indwelling intravenous catheters. Methodologic aspects. Haemostasis 4: 40
12 McFarlane AS. 1963; In vivo behavior of I¹³¹-fibrinogen. Journal of Clinical Investigation 42: 346
13 Müller-Berghaus G, Moelller RM, Mahn I. 1977. Untersuchungen zur Fibrinogenkinetik bei trächtigen Kaninchen. 21 Tagung der Deutschen Arbeitsgemeinschaft für Blutgerinnungsforschung; Essen:
14 Müller-Berghaus G, Moeller RM, Mahn I. 1977. Fibrinogen turnover in pregnant rabbits during the first and the last third of gestation. Submitted for publication
15 Nilsson IM, Kullander S. 1967; Coagulation and fibrinolytic studies during pregnancy. Acta Obstetrica et Gynecologica Scandinavica 46: 273
16 Regoeczi E. 1970. Abnormal fibrinogen metabolism. In: Rothschild MA, Waldmann TA. (eds.) Plasma Protein Metabolism. Regulation of Synthesis, Distribution and Degradation. Academic Press; New York: 459
17 van Royen EA, Ten Cate JW. 1976; Generation of a thrombin-like activity in late pregnancy. Thrombosis Research 8: 487
18 Sachs L. Angewandte Statistik. 4th edn Springer-Verlag, Berlin, Heidelberg; New York:
19 Straub PW. 1976. Zirkulierendes Fibrin und Hyperkoagulabilität. In: Lüscher EF. (ed.) Die Rolle der Blutplattchen bei der Thrombose. Das Problem der Hyperkoagulabilität. Schattauer Verlag; Stuttgart – New York: 95
20 Swank RT, Munkres KD. 1971; Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate. Analytical Biochemistry 39: 462