Sensitive Methods for the Detection and Characterization of Platelet Isoantibodies

Richard J. Hirschman; Ronald A. Yankee; Barry S. Coller; Harvey R. Gralnick

doi:10.1055/s-0038-1647781

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 29(02): 408-415
DOI: 10.1055/s-0038-1647781

Original Article

Schattauer GmbH

Sensitive Methods for the Detection and Characterization of Platelet Isoantibodies

Richard J. Hirschman

¹Hematology Service, Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Maryland and the Hematology and Supportive Care Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

,

Ronald A. Yankee^*

¹Hematology Service, Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Maryland and the Hematology and Supportive Care Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

,

Barry S. Coller

¹Hematology Service, Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Maryland and the Hematology and Supportive Care Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

,

Harvey R. Gralnick

¹Hematology Service, Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, Maryland and the Hematology and Supportive Care Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

› Author Affiliations

Abstract

Summary

Two sensitive methods for the detection and characterization of platelet isoantibodies are presented in this paper. The methods involve incubating platelets with either radioactive serotonin or adenine, interaction of the platelets with serum antibody and finally detection of supernatant radioactivity. Both tests can be used for HL-A typing of platelets and both tests detect antibodies undetectable by complement fixation. The requirement of a heat labile serum component is variable in the serotonin test but absolute in the adenine test. Antibody activity was found to be in the 7S gamma globulin fraction on DEAE cellulose column chromatography.

Full Text

References

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