Comparative Evaluation of a Platelet Serotonin Assay Utilizing Ortho-Phthaldialdehyde for Fluorophore Production

Thromb Haemost 1975; 33(01): 130-131
DOI: 10.1055/s-0038-1647825

Letter to the Editor

Schattauer GmbH

Jonathan L Costa

¹Laboratory of Clinical Science Section on Clinical Neuropharmacology NIMH Bethesda, Md. 20014

,

J. Steven Richardson

¹Laboratory of Clinical Science Section on Clinical Neuropharmacology NIMH Bethesda, Md. 20014

,

Dennis L Murphy

¹Laboratory of Clinical Science Section on Clinical Neuropharmacology NIMH Bethesda, Md. 20014

References
1 Bogdanski B. F, Pletscher A, Brodie B. B, Udenfriend S. Identification and assay of serotonin in brain. Journal of Pharmacology and Experimental Therapeutics 1956; 117: 82
2 Costa J. L, Reese T. S, Murphy D. L. Serotonin storage in platelets: estimation of storage-packet size. Science 1974; 183: 537
3 Crosti P. F, Lucchelli P. E. An easy method to determine the serotonin content of human platelets. Journal of Clinical Pathology 1962; 15: 191
4 Drummond A. H, Gordon J. L. Rapid sensitive microassay for platelet 5HT. Thrombosis et Diathesis Haemorrhagica 1974; 31: 366
5 Komesu N. N, Thompson J. H. Optimal concentration of orthophthaldialdehyde for the fluorometric measurement of serotonin. European Journal of Pharmacology 1971; 16: 248
6 Saavedra J. M, Brownstein M, Axelrod J. A specific and sensitive enzymatic-isotopic microassay for serotonin in tissues. Journal of Pharmacology and Experimental Therapeutics 1973; 186: 508
7 Snyder S. H, Axelrod J, Zweig M. A sensitive and specific fluorescence assay for tissue serotonin. Biochemical Pharmacology 1965; 14: 831