Assay of Brinase Inhibitors with Soluble and Insoluble Substrates

D Nyman; F Duckert

doi:10.1055/s-0038-1647875

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1975; 33(02): 221-225
DOI: 10.1055/s-0038-1647875

Original Article

Schattauer GmbH

Assay of Brinase Inhibitors with Soluble and Insoluble Substrates

Authors

D Nyman

¹Coagulation and Fibrinolysis Laboratory of the Dept. of Internal Medicine, University of Basle, Switzerland
F Duckert

¹Coagulation and Fibrinolysis Laboratory of the Dept. of Internal Medicine, University of Basle, Switzerland

Abstract

Summary

Two methods for the assay of brinase inhibitors are compared. Neither provided a reliable means of brinase dose prediction. Overdosage can, however, be avoided by preinfusion inhibitor assay.

Full Text

References

References
1 Amundsen E, Svendsen L, Vefling A. A sensitive photometric method for the determination of plasma inhibition towards plasmin and brinase. IVth International Congress on Thrombosis and Hemostasis; Vienna: Abstr. 1973: 54
2 Bergkvist R. The proteolytic enzymes of Aspergillus oryzae IV On the inhibition of the enzymes by serum. Acta Chemica Scandinavica 1963; 17: 2239
3 Clauss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta Haematologica 1957; 17: 235
4 Fitzgerald D. E, Frisch E. P. Relief of chronic peripheral artery obstruction by intravenous brinase. Journal of the Irish Medical Association 1973; 66: 3
5 Freedman H. J, Roschlau W. H. E. Some factors influencing the toxicity of thrombolytic enzymes (brinase). Canadian Journal of Physiology and Pharmacology 1970; 48: 69
6 Frisch E. P, Blombäck M, Ekeström D, Kiessling H, Lund F, Magaard F. Dosage of brinase based on determination of brinase inhibitors and coagulation studies in 17 patients treated with the enzyme. To be published 1975
7 Kiessling H. Personal communications 1973
8 Lund F, Ekeström S, Frisch E. P, Magaard F. Thrombolytic treatment with brinase of peripheral obliterative arterial disease with ischemic skin lesions. Lecture at the Annual Meeting of the Swedish Medical Association 1973: 29 November
9 Nyman D, da Silva M. A, Widner L. K, Duckert F. Thrombolytische Behandlung arterieller Verschlüsse mit Brinase, einem proteolytischen Enzym aus Aspergillus. Schweizerische medizinische Wochenschrift 1974; 104: 1865
10 Roschlau W. H. E. The evaluation in the dog of a proteolytic enzyme derived from Aspergillus oryzae. Canadian Journal of Physiology and Pharmacology 1964; 42: 109
11 Svaerd P. O, Bergkvist R. Unpublished results 1962
12 Wissenschaftliche Tabellen 7. Edition J. R. Geigy A.G., Pharma, Basel 1968; 552