The Kinin System of Human Plasma

Ayşe H. Özge-Anwar; H. Z Movat; J. G Scott

doi:10.1055/s-0038-1649349

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1972; 27(01): 141-158
DOI: 10.1055/s-0038-1649349

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

The Kinin System of Human Plasma

IV. The Interrelationship between the Contact Phase of Blood Coagulation and the Plasma Kinin System in Man^[*]

Authors

Ayşe H. Özge-Anwar

¹The Division of Experimental Pathology, Department of Pathology, the Institute of Immunology and the Division of Hematology, Toronto General Hospital, Department of Medicine University of Toronto - Toronto, Canada
H. Z Movat^**

¹The Division of Experimental Pathology, Department of Pathology, the Institute of Immunology and the Division of Hematology, Toronto General Hospital, Department of Medicine University of Toronto - Toronto, Canada
J. G Scott

¹The Division of Experimental Pathology, Department of Pathology, the Institute of Immunology and the Division of Hematology, Toronto General Hospital, Department of Medicine University of Toronto - Toronto, Canada

Abstract

Summary

Human plasma was adsorbed to celite. The celite eluates were chromatographed on QAE-Sephadex A-50, followed by gel filtration, isoelectric focusing and preparative polyacrylamide electrophoresis.

By anion-exchange chromatography, factor XI a, kallikrein and y-globulin were isolated from the excluded protein peak. Factor XIa (MW ∼170,000) corrected the deficiency in plasmas deficient in factors XII or XI, but had no effect on the kininsystem. Its isoelectric point was about 8.0-8.5. Kallikrein had a molecular weight of ∼ 90,000 and a pi of 7.8-8.0 The two substances could be isolated also by polyacrylamide electrophoresis.

Highly anionic fractions contained a fragment of factor XII a (prekallikrein activator), which activated prekallikrein and retained some of the clot-promoting property of the intact factor XIIa molecule. Its molecular weight was about 37,000, the pi about 4.4 and it migrated as a prealbumin by disc electrophoresis.

It is concluded that, by massive contact exposure, most of factor XII a converts to the highly anionic, low-molecular-weight prekallikrein activator. Since the prekallikrein activator is a fragment of factor XII a, we recommend that it be referred to as “XII f”.

Full Text

References

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