Induction of Endothelial Tissue Factor by Endotoxin and Its Precursors

Charles F Moldow; Ronald R Bach; Katherine Staskus; Paul D Rick

doi:10.1055/s-0038-1649653

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1993; 70(04): 702-706
DOI: 10.1055/s-0038-1649653

Original Article

Von Willebrand Factor And Endothelial Cells

Schattauer GmbH Stuttgart

Induction of Endothelial Tissue Factor by Endotoxin and Its Precursors

Charles F Moldow

¹The Department of Medicine, Minneapolis VA Medical Center, Minnesota, USA,

,

Ronald R Bach

¹The Department of Medicine, Minneapolis VA Medical Center, Minnesota, USA,

,

Katherine Staskus

²Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA

,

Paul D Rick

³Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA

› Author Affiliations

Abstract

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Summary

The structural determinants of lipopolysaccharide required for the induction of tissue factor in human umbilical vein endothelial cells were studied. Intact lipid A was essential for the induction of tissue factor whereas the incomplete lipid A precursors lipid IV_A and lipid X, as well as monophosphoryl lipid A and acyloxyacyl hydrolase-treated lipopolysaccharide, were unable to induce tissue factor and tissue factor specific mRNA. However, the lipid A precursor, lipid IV_A, was able to inhibit LPS-mediated induction of tissue factor; structural determinants distal to lipid A were found to be required for maximal induction of tissue factor activity and tissue factor mRNA. The presence of serum in the assay was found to amplify but was not obligate for tissue factor induction by LPS.

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References

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