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Thromb Haemost 1989; 62(03): 1000-1005
DOI: 10.1055/s-0038-1651042
Original Article
Schattauer GmbH Stuttgart

Ouabain Enhances Basal and Stimulus-Induced Cytoplasmic Calcium Concentrations in Platelets

A D Lees
The University Department of Medicine, The General Infirmary, Leeds, UK
,
J Wilson
The University Department of Medicine, The General Infirmary, Leeds, UK
,
C H Orchard
*   The Department of Physiology, University of Leeds, Leeds, UK
,
M A Orchard
The University Department of Medicine, The General Infirmary, Leeds, UK
› Author Affiliations
Further Information

Publication History

Received 29 September 1988

Accepted after revision 13 July 1989

Publication Date:
30 June 2018 (online)

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Summary

Incubation of platelet-rich plasma (PRP) with ouabain, an inhibitor of sodium/potassium ATPase (Na+/K+ ATPase), induced a significant rise in basal platelet intracellular calcium concentration ([Ca2+]i) when measured using fura 2. Ouabain induced an enhanced aggregation response to low doses of collagen in both PRP and washed platelets loaded with aequorin. In aequorin loaded platelets this enhanced aggregation response was associated with an enhanced rise in [Ca2+]j such that the relationship between [Ca2+]i and aggregation was unchanged. As inhibition of plasma membrane Na+/K+ ATPase would lead to a raised intracellular sodium ion concentration ([Na+]i) the results suggest that in the platelet, [Na+]i can modulate [Ca2+]i and hence influence the response of platelets to stimuli such as collagen.

Keywords

Platelets - Ouabain - Ca2+ - Na+
 

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