Summary
An evaluation of changes in the plasma chromatographic pattern was carried out in
dogs with experimental coronary thrombosis. Sequential samples were taken simultaneously
from the aorta and coronary sinus over a two, four and twenty-four hour period. In
addition to gel-chromatography, immunodiffusion and electrophoresis were also employed
to identify the protein components of the elution fractions. The results showed that
there was a shift of the normal chromatographic pattern to the left from the control
within two hours following thrombotic occlusion of the coronary artery and it was
maintained over a twenty-four hour period. The shift to the left was presumably due
to the earlier elution of the heavy molecular weight components forming as a result
of the thrombotic process. The early appearance of the altered chromatographic pattern
constitutes an important advantage worthy of further exploration for possible diagnostic
application in the human subject.
