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Thromb Haemost 1981; 46(03): 634-637
DOI: 10.1055/s-0038-1653435
Original Article
Schattauer GmbH Stuttgart

Studies of Phospholipid Reagents Used in Coagulation II: Factors Influencing Their Sensitivity to Heparin

T W Barrowcliffe
The National Institute for Biological Standards and Control, Holly Hill, Hampstead, London, U. K
,
Elaine Gray
The National Institute for Biological Standards and Control, Holly Hill, Hampstead, London, U. K
› Author Affiliations
Further Information

Publication History

Received 26 May 1981

Accepted 07 August 1981

Publication Date:
05 July 2018 (online)

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Summary

This study demonstrates the many technical variables which exist in the use of APTT reagents to detect and measure heparin. With the same phospholipid reagent different activation regimes produced significant differences in heparin sensitivity. The concentration of phospholipid was also an important influence; decreasing concentration led to increasing sensitivity, and the highest sensitivity was given by omitting the phospholipid altogether. Other factors which influenced the sensitivity to heparin were buffering of the reagents and the presence of oxidation products in the phospholipid. The results emphasize the need for standardisation of at least some of these variables if the APTT is to be used to determine the therapeutic range of heparin.

Keywords

Heparin - Phospholipids - APTT
 

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