Summary
Platelet aggregation was measured by Born’s method. Plasma from Sprague-Dawley rats
and inbred albino rabbits was used. 20% ethanol in 0.9%(w/v) NaCl was added to plasma
at final concentrations of 56.5 mM, 85.9 mM 171.5 mM and 343.1 mM or to whole blood
at final concentrations of 8.7 mM, 17.4 mM, 52.5 mM, 171.5 mM and 343.1 mM. Experiments
using 0.9% NaCl volumes equivalent to the added ethanol volume were also conducted.
Dose-response aggregation tests showed that a decrease in ADP-and collagen-induced
aggregation existed when ethanol was mixed with rat or rabbit blood prior to the preparation
of platelet-rich plasma. There was no effect on ADP-induced platelet aggregation when
ethanol was mixed with rat or rabbit plasma. Collagen-induced aggregation was impaired
only in rat plasma when ethanol concentration reached 343.1 mM. These results suggested
that ethanol modified platelet function possibly via red cells.
Keywords
Ethanol - Platelet rich plasma - Blood - Platelet aggregation
