Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1962; 08(01): 112-120DOI: 10.1055/s-0038-1655417 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Methoden zur fluorimetrischen Bestimmung der fibrinolytischen Aktivität Authors Author Affiliations R Strässle 1 Chemische Forschungsabteilung der F. Hoffmann-La Roche & Co. A.G., Basel Recommend Article Abstract PDF Download(opens in new window) Buy Article(opens in new window) Zusammenfassung Es werden zwei Methoden vorgeschlagen, welche erlauben, unter Verwendung von Fluorescein-markiertem Rinderfibrin als Substrat die fibrinolytische Totalaktivität biologischer Flüssigkeiten in kurzer Zeit mit einem relativen Fehler von etwa ± 2% zu ermitteln. Die Methoden lassen sich, den Erfordernissen entsprechend, modifizieren. PDF (9455 kb) References Literatur 1 Astrup T. Fibrinolysis in the organism.. Blood 1956; 11: 781 2 Sherry S, Fletcher A, Alkjaersig N. Fibrinolysis and fibrinolytic activity in man.. Physiol. Revs. 1959; 39: 343 3 Symposium on fibrinolysis, Sloan-Kettering Institute for Cancer Research, 25./26. 3. 1960.. Panel Discussion Assay Technics. Am. J. Cardiol. 1960; 6: 447 4 Jürgens J, Beller F. K. Klinische Methoden der Blutgerinnungsanalyse. Bestimmung des fibrinolytischen Systems.. G. Thieme, Stuttgart, S. 1959; 239 5 Perlick E. Gerinnungslaboratorium in Klinik und Praxis.. Nachweis der Fibrinolyse-Aktivität. G. Thieme, Leipzig, S. 209 1960 6 Stamm H. Einführung in die Klinik der Fibrinolyse.. Die Messung der fibrinolytischen Aktivität. S. Karger, Basel, S. 1962: 166 7 Shulman N. R, Tagnon N. J. Proteolytic activity determined with a substrate tagged with radioactive iodine.. J. Biol. Chem. 1950; 186: 69 8 Shulman N. R. Studies on the inhibition of proteolytic enzymes by serum. I. The mechanism of the inhibition of trypsin, plasmin, and chymotrypsin by serum using fibrin tagged with I131 as a substrate.. J. exp. Med. 1952; 95: 571 9 Alkjaersing N, Fletcher A. P, Sherry S. The mechanism of clot dissolution by plasmin.. J. Clin. Invest. 1959; 38: 1086 10 Lüscher E, Käser-Glanzmann R. A new method for the quantitative determination of fibrinolytic activities, based on the use of fluorescent fibrin as a substrate.. Vox Sanguinis 1961; 6: 116 11 Cohn E. J, Strong L. E, Hughes jr. W. L, Mulford D. J, Ash-worth J. N, Melin M, Taylor H. L. Preparation and properties of serum and plasma proteins. IV. A system for the separation into fractions of the protein and lipoprotein components of biological tissues and fluids.. J. Am. Chem. Soc. 1946; 68: 459 12 Blombäck B, Blombäck M. Purification of human and bovine fibrinogen.. Arkiv för Kemi 1956; 10: 415 13 Blombäck B. On the properties of fibrinogen and fibrin.. Arkiv for Kemi 1958; 12: 99 14 Mosesson M. W. The preparation of human fibrinogen free of plasminogen.. Biochim. Biophys. Acta 1962; 57 (02) 204 15 Vignal A, Blatrix Ch, Steinbuch M. Fibrinogen free of plasminogen as substrate for fibrinolytic assays.. Nature 1962; 193 4816 693 16 Riggs J. L, Seiwald R. J, Burckhalter J. H, Downs C. M, Metcalf T. G. Isothiocyanate compounds as fluorescent labeling agents for immune serum.. Am. J. Pathol. 1958; 34: 1081 17 George W, Walton K. W. Purification and concentration of dye-protein conjugates by gel filtration.. Nature 1961; 192: 1188 18 Lipp W. Use of gel filtration and polyethylene glycol in the preparation of fluoro-chrome-labelled proteins.. J. Histochem. Cytochem. 1961; 9: 458 19 Curtain C. C. The chromatographic purification of fluorescein-antibody.. J. Histochem. Cytochem. 1961; 9: 484 20 Killander J, Pontén J, Roden L. Rapid preparation of fluorescent antibodies using gel-filtration.. Nature 1961; 192: 182 21 Müllertz S, Lassen M. An activator system in blood indispensable for formation of plasmin by streptokinase.. Proc. Soc. exp. Biol. Med. 1953; 82: 264
