Molecular and Genetic Analysis of Two Patients with Bernard-Soulier Syndrome – Identification of New Mutations in Glycoprotein Ibα Gene

Taisuke Kanaji; Takashi Okamura; Mika Kuroiwa; Masaaki Noda; Kingo L Fujimura; Atsushi Kuramoto; Masayuki Sano; Shuji Nakano; Yoshiyuki Niho

doi:10.1055/s-0038-1656111

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1997; 77(06): 1055-1061
DOI: 10.1055/s-0038-1656111

Clinical Studies

Schattauer GmbH Stuttgart

Molecular and Genetic Analysis of Two Patients with Bernard-Soulier Syndrome – Identification of New Mutations in Glycoprotein Ibα Gene

Authors

Taisuke Kanaji

¹The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
Takashi Okamura

¹The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
Mika Kuroiwa

¹The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
Masaaki Noda

¹Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, . Hiroshima, Japan
Kingo L Fujimura

¹Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, . Hiroshima, Japan
Atsushi Kuramoto

¹Department of Internal Medicine, Research Institute for Nuclear Medicine and Biology, Hiroshima University, . Hiroshima, Japan
Masayuki Sano

³Department of Transfusion Medicine, Saga Medical School, Saga, Japan
Shuji Nakano

¹The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan
Yoshiyuki Niho

¹The First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan

Abstract

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Summary

We investigated two unrelated patients with Bernard-Soulier syndrome (BSS) by performing molecular and genetic analysis.

A flow cytometric and immunoblotting analysis showed GP Ibα to be absent from the platelet membrane of both patients. Other glycoproteins that formed GP Ib/IX/V complex were present on the platelets, but in decreased amounts. Therefore, GP Ibα gene from both cases was sequenced after PCR amplification and subcloning. We identified a homozygous mutation of a dinucleotide deletion within the TGTG repeat at cDNA number 972 to 975 in GP Ibα gene from Case 1. In Case 2, compound heterozygosity was demonstrated in GP Ibα gene; an insertion of a single base (T) at cDNA number 1,418 in one allele, and a deletion of a single base (A) within the 7-adenine repeat at cDNA number 1,438 to 1,444 in another allele. The three new mutations in both patients appeared to cause a frameshift, which created a new termination codon shortly thereafter, and thus lead to a GP Ibα deficiency on the platelet membrane. Truncated mutant proteins could be detected in the plasma and platelets of Case 2, but not of Case 1. According to these findings, it is thus supposed that the properties and conformation of additional COOH-terminal peptides, which were supposedly synthesized as results of the mutations, may have an important role on the processing of mutant GP Ibα in megakaryocytes and platelets.

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References

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