Kinetic Aspects of the Interaction of Blood Clotting Enzymes

H. C Hemker; P.W Hemker; E. A Loeliger

doi:10.1055/s-0038-1656222

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1965; 13(01): 155-175
DOI: 10.1055/s-0038-1656222

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH Schattauer

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

I. Derivation of Basic Formulas

H. C Hemker

¹Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.

,

P.W Hemker

¹Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.

,

E. A Loeliger

¹Haematology Section of the Department of Internal Medicine (Head: Prof .J .Mulder, M.D.), University H ospita I, and the Thrombosis Service (Head: E.A.Loeliger, M.D.), Ley den, The Netherlands.

› Author Affiliations

Abstract

Summary

Application of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :

1. Substrate is not present in excess.

2. Only relative measures exist for concentrations of substrate or enzymes.

3. Enzymes and substrates are often added together.

4. Reagents are not pure.

5. Clotting-time is our only measure for clotting-velocity.

Formulas are deduced, which makes it possible to recognize the effect of these complications.

Full Text

References

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