Summary
A new method is described for the preparation of highly purified human plasminogen
and plasmin with specific activity of 32 CTA units per mg of protein. With this method,
the purification of the urinary plasminogen + plasmin antigenic materials from patients
with chronic glomerulonephritis, disseminated intravascular coagulation syndrome and
severe toxemia of pregnancy was performed, and the resulting highly purified proenzyme
and enzyme were analyzed by immunoelectrophoresis, separative agar electrophoresis,
gel filtration and SDS-gel electrophoresis.
Our findings indicated that urinary plasmin reflects more closely the extent of intraglomerular
fibrinolysis, while urinary plasminogen reflects non-selective proteinuria in patients
with chronic glomerulonephritis or severe toxemia of pregnancy.
