Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1997; 78(01): 577-580DOI: 10.1055/s-0038-1657592 Thrombin and its modulation Schattauer GmbH Stuttgart Modulation of Thrombin’s Procoagulant and Anticoagulant Properties Authors Author Affiliations Lawrence L K Leung 1 Division of Hematology, Department of Medicine, Stanford University Medical School, Stanford, CA, USA Craig S Gibbs 2 Gilead Sciences, Foster City, CA, USA Recommend Article Abstract PDF Download(opens in new window) Buy Article(opens in new window) PDF (632 kb) References References 1 Esmon CT. Thrombomodulin as a model of molecular mechanisms that modulate protease specificity and function at the vessel surface. FASEB J 1995; 9: 946-955 2 Dang QD, Vindigni A, Di Cera E. An allosteric switch controls the procoagulant and anticoagulant activities of thrombin. Proc Natl Acad Sci USA 1995; 92: 5977-5981 3 Wells CM, Di Cera E. Thrombin is a Na(+)-activated enzyme. Biochemistry 1992; 31: 11721-11730 4 Di Cera E, Guinto ER, Vindigni A, Dang QD, Ayala YM, Wuyi M, Tulinsky A. The Na+ binding site of thrombin. J Biol Chem 1995; 270: 22089-22092 5 Dang QD, Di Cera E. Residue 225 determines the Na+-induced allosteric regulation of catalytic activity in serine proteases. Proc Natl Acad Sci USA 1996; 93: 10653-10656 6 Wu Q, Sheehan JP, Tsiang M, Lentz SR, Birktoft JJ, Sadler JE. Single amino acid substitutions dissociate fibrinogen-clotting and thrombomodulin-binding activities of human thrombin. Proc Natl Acad Sci USA 1991; 88: 6775-6779 7 Tsiang M, Jain AK, Dunn KE, Rojas ME, Leung LLK, Gibbs CS. Functional mapping of the surface residues of thrombin. J Biol Chem 1995; 270: 16854-16863 8 Tsiang M, Jain AK, Gibbs CS. Functional requirements for inhibition of thrombin by antithrombin III in the presence and absence of heparin. Submitted for publication. 9 Sheehan JP, Sadler JE. Proc Natl Acad Sci USA 1994; 91: 5518-5522 10 Gan ZR, Li Y, Chen Z, Lewis SD, Shafer JA. J Biol Chem. 1994; 269: 1301-1305 11 Gibbs CS, Coutre SE, Tsiang M, Li WX, Jain AK, Dunn KE, Law VS, Mao CT, Matsumura SY, Mejza SJ, Paborsky LR, Leung LLK. Conversion of thrombin into an anticoagulant by protein engineering. Nature 1995; 378: 413-416 12 Tsiang M, Paborsky LR, Li WX, Jain AK, Mao CT, Dunn KE, Lee DW, Matsumura SY, Matteucci M, Coutre SE, Leung LLK, Gibbs CS. Protein engineering thrombin for optimal specificity and potency of anticoagulant activity in vivo. Biochemistry 1996; 35: 16449-16457 13 Musci G, Berliner LJ. Ligands which effect human protein C activation by thrombin. J Biol Chem 1987; 262: 13889-13891 14 Berg DT, Wiley MR, Grinnell BW. Enhanced protein C activtion and inhibition of fibrinogen cleavage by a thrombin modulator. Science 1996; 273: 1389-1391 15 Solis MM, Vitti M, Cook J, Young D, Glaser C, Light D, Morser J, Wydro R, Yu S, Fink L. et al Recombinant soluble human thrombomodulin: a randomized, blinded assessment of prevention of venous thrombosis and effects on hemostatic parameters in a rat model. Thromb Res 1994; 73: 385-394 16 McBane RD, Wysokinski WE, Chesebro JH, Owen WG. Antithrombotic action of endogenous porcine protein C activated with a latent porcine thrombin preparation. Thromb. Haemostasis 1995; 74: 879-885
