Hepatic Clearance of Tissue-Type Plasminogen Activator in Rats

J J Emeis; C M van den Hoogen; D Jense

doi:10.1055/s-0038-1660092

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 54(03): 661-664
DOI: 10.1055/s-0038-1660092

Original Article

Schattauer GmbH Stuttgart

Hepatic Clearance of Tissue-Type Plasminogen Activator in Rats

Authors

J J Emeis

The TNO Gaubius Institute for Cardiovascular Research, Leiden, The Netherlands
C M van den Hoogen

The TNO Gaubius Institute for Cardiovascular Research, Leiden, The Netherlands
D Jense

The TNO Gaubius Institute for Cardiovascular Research, Leiden, The Netherlands

Abstract

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Summary

The clearance of tissue-type plasminogen activator (t-PA) was studied, in rats, by use of a functional assay for t-PA. Half-lives in the circulation were about one minute both for human (melanoma cell-derived) t-PA and for the rat’s own t-PA. The clearance of t-PA required an intact liver blood flow. In isolated liver perfusion experiments the hepatic extraction of t-PA did not require any plasma proteins, including fast-acting t-PA inhibitor. Competition experiments, using monosaccharides, suggested that known hepatic glycoprotein receptors were not involved in hepatic t-PA extraction.

Keywords

Rat - Tissue-type plasminogen activator - Clearance - Liver - Carbohydrate - Tunicamycin

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References

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