The Calibration of the Second Primary International Reference Preparation for Thromboplastin (Thromboplastin, Human, Plain, Coded BCT/253)

Jean M Thomson; J A Tomenson; L Poller

doi:10.1055/s-0038-1661209

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1984; 52(03): 336-342
DOI: 10.1055/s-0038-1661209

Original Article

Schattauer GmbH Stuttgart

The Calibration of the Second Primary International Reference Preparation for Thromboplastin (Thromboplastin, Human, Plain, Coded BCT/253)

Authors

Jean M Thomson

The UK Reference Laboratory for Anticoagulant Reagents and Control, Withington Hospital, Manchester, UK
J A Tomenson

The UK Reference Laboratory for Anticoagulant Reagents and Control, Withington Hospital, Manchester, UK
L Poller

The UK Reference Laboratory for Anticoagulant Reagents and Control, Withington Hospital, Manchester, UK

Abstract

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Summary

An international collaborative exercise has been undertaken to calibrate a replacement for the first WHO primary international reference preparation (IRP) for thromboplastin. The replacement preparation is a lyophilised batch of British Comparative Thromboplastin (BCT/253, human plain) for use in the Quick prothrombin time test. Seventeen centres participated. The experimental design, calibration model and statistical analysis were based on the recommended WHO procedure. As a result of this calibration exercise an International Sensitivity Index (ISI) of 1.1 has been assigned to the preparation by WHO and it has been officially recognised as the second primary IRP for thromboplastin.

The calibration of BCT/253 is an essential link in a new hierarchical structure for the standardisation of the prothrombin time. The aim is to provide a uniform international system of reporting prothrombin time results using International Normalised Ratios (INR) derived from the ISI of individual thromboplastins.

Key words

International reference thromboplastin - Thromboplastin calibration - Prothrombin time standardisation

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References

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