Summary
Native human plasminogen, the proenzyme of plasmin (E. C. 3.4.21.7) occurs in blood
in two well defined forms, affinity forms I and II. In this paper, the feasibility
of separating these forms of human native plasminogen by affinity chromatography,
is shown to be dependent on two factors: 1) the ionic composition of the buffer containing
the displacing agent: buffers of varying contents of sodium, Tris, phosphate and chloride
ions were compared, and 2) the type of adsorbent. Two adsorbents were compared: Sepharose-lysine
and Sepharose-bisoxirane-lysine. Only in the phosphate containing buffers, irrespective
of the type of adsorbent, the affinity forms can be separated. The influence of the
adsorbent can be accounted for by a large difference in dissociation constants of
the complex between plasminogen and the immobilized lysine.
Key words
Plasminogen affinity forms - Affinity chromatography
