IgG Binding to Endothelial Cells in Systemic Lupus Erythematosus

Summary

Endothelial-asSociated IgG were determined in 20 patients with systemic lupus erythematosis (SLE) - 8 of whom had a lupus anticoagulant (LA) and 6 a history of thrombosis. The binding of IgG present in patient plasma to cultured human endothelial cells was detected using radiolabeled staphylococcal protein A. Thirteen samples gave positive results and a significant association between endothelial-associated IgG and lupus anticoagulant was found (p <0.05). No statistically significant relationship with a previous history of thrombosis was found. These results suggest that the lupus anticoagulant may be directly involved in immune vascular injury induced by either antibodies or immune complexes in SLE.

Key words

SLE - Antiendothelial cells antibodies - Thrombosis and lupus anticoagulant

• References

• 1 Boey ML, Colaco CB, Gharavi AE, Elkon KB, Loizou S, Hugues GR V. Thrombosis in systemic lupus erythematosus: striking association with the presence of circulating lupus anticoagulant. Br Med J 1983; 287: 1021-1023
• 2 Bowie EJ W, Thompson JH, Pascuzzi CA. Thrombosis in systemic lupus erythematosus despite circulating anticoagulants. J Lab Clin Med 1963; 62: 416-430
• 3 Carreras LO, Vermylen JG. "Lupus" anticoagulant and thrombosis. Possible role of inhibition of prostacyclin formation. Thromb Haemos-tas 1982; 48: 38-40
• 4 Elias M, Eldor A. Thromboembolism in patients with the "lupus" -type circulating anti-coagulant. Arch Intern Med 1984; 144: 510-515
• 5 Gastineau DA, Kazmier FJ, Nichols WL, Bowie EJ W. Lupus anticoagulant: An analysis of the clinical and laboratory features of 219 cases. Am J Hematol 1985; 19: 265-275
• 6 Gladman DD, Urowitz MB, Tozman EC, Glynn MF. Haemostatic abnormalities in systemic lupus erythematosus. Quaterly J Med 1983; Lib 424-433
• 7 Mueh JR, Herbst KD, Rapaport SI. Thrombosis in patients with the lupus anticoagulant. Ann Intern Med 1980; 92: 156-159
• 8 Peck B, Hoffman GS, Franck WA. Thrombophlebitis in systemic lupus erythematosus. Jama 1978; 204: 1728-1730
• 9 Carreras LO, Defreyn G, Machin SJ. Arterial thrombosis, intrauterine death and "lupus" anticoagulant: detection of immunoglobulin interfering with prostacyclin formation. Lancet 1981; 1: 244-246
• 10 McVerry BA, Machin SJ, Parry H, Goldstone AH. Reduced prostacyclin activity in systemic lupus erythematosus. Ann Rheum Dis 1980; 39: 524
• 11 Cariou R, Tobelemyg G. Effet des anticoagulants du type &lupus& sur 1’ activation de la proteine C. Responsabilite dans la survenue des thromboses? 13eme Congres National de Medecine Interne, Dijon, France. 1985 (in press)
• 12 Comp PC, Debault LE, Esmon NL, Esmon CT. Human thrombomodulin is inhibited by IgG from two patients with nonspecific anticoagulants. Blood 1983; (Suppl. 01) 1099
• 13 Cines DB, Lyss AP, Reeber M, Bina M, Dehoratius RJ. Presence of complement fixing anti-endothelial cell antibodies in systemic lupus erythematosus. J Clin Invest 1984; 73: 611-625
• 14 Tan EM, Cohen AS, Fries JF. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthr Rheum 1982; 25: 1271-1277
• 15 Kelsey PR, Stevenson KJ, Poller L. The diagnostis of lupus anticoagulants by the activated partial thromboplastin time. The central role of phosphatidyl serine. Thromb Haemostas 1984; 52: 172-175
• 16 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-2756
• 17 Howard MA, Firkin BG. Investigations of the lupus-like inhibitor bypassing activity of platelets. Thromb Haemostas 1983; 50: 775-779
• 18 Lafer EM, Rauch J, Andrze-Jewski C. Polyspecific monoclonal lupus auto-antibodies reactive with both polynucleotides and phospholipids. J Exp Med 1981; 153: 897-909
• 19 Shoenfeld Y, Rauch J, Massicotte H. Polyspecificity of monoclonal lupus auto antibodies produced by human hybridomas. N Engl J Med 1983; 308: 414-420
• 20 Lubbe WF, Butler WS, Palmer SJ, Liggins GC. Lupus anticoagulant in pregnancy. Br J Obstet Gynaecol 1984; 91: 357-363
• 21 Prentice RL, Gatenby PA, Loblay RH, Shearman RP, Kronenberg H, Basten A. Lupus anticoagulant in pregnancy. Lancet 1984; 2: 464
• 22 Soulier JP, Boffa MA. Avortements a repetition, thromboses, anticoagulant circulant antithromboplastine. Nouv Presse Med 1980; 9: 859-865
• 23 Harris EN, Asherson RA, Gharavi AE, Morgan SH, Derue G, Hugues GR V. Thrombocytopenia in SLE and related autoimmune disorders: association with anticardiolipin antibody. Br J Haematol 1985; 59: 227-230
• 24 Harris EN, Gharavi AE, Boey ML, Patel BM, Mackworth-Young CG, Loizou S, Hugues GR V. Anticardiolipin antibodies: detection by jradioimmuno assay and association with thrombosis in systemic lupus erythematosus. Lancet 1983; 2: 1211-1213
• 25 Freyssinet JM, Brami B, Gauchy J, Cazenave JP. Coextraction of thrombomodulin and tissue factor from human placenta: effects of concanavalin A and phospholipid environment on activity. Thromb Haemostas 1986; 55: 112-118
• 26 Angles-Cano E, Sultan Y, Clauvel J. Predisposing factors to thrombosis in systemic lupus erythematosus. J Lab Clin Med 1979; 94: 312-323
• 27 Cines DB, Lyss PA, Bina M, Corkey R, Kefalides NA. Fc and C3 receptors induced by herpes simplex virus on cultured human endothelial cells. J Clin Invest 1982; 69: 123-128
• 28 Ryan US, Schultz DR, Ryan JW. Fc and C3b receptors on pulmonary endothelial cells: induction by injury. Science 1981; 214: 557-558