The Influence of Thrombin on the Platelet-Aggregating Activity of Human Factor VIII. A Study with Insolubilized Thrombin

Th Vukovich; E Koller; W Doleschel

doi:10.1055/s-0038-1666931

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1979; 42(03): 832-837
DOI: 10.1055/s-0038-1666931

Original Article

Schattauer GmbH Stuttgart

The Influence of Thrombin on the Platelet-Aggregating Activity of Human Factor VIII. A Study with Insolubilized Thrombin

Authors

Th Vukovich

The Department of Medical Physiology, School of Medicine, University of Vienna, Schwarzspanierstr. 17, A-1090, Vienna, Austria
E Koller

The Department of Medical Physiology, School of Medicine, University of Vienna, Schwarzspanierstr. 17, A-1090, Vienna, Austria
W Doleschel

The Department of Medical Physiology, School of Medicine, University of Vienna, Schwarzspanierstr. 17, A-1090, Vienna, Austria

Abstract

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Summary

A study was made of the influence of thrombin on the platelet-aggregating activity of human factor VIII with Ristocetin as cofactor. Purified factor VIII and different amounts of a thrombin-Sepharose 2B complex were mixed and incubated for various periods of time. The factor VIII-related platelet-aggregating activities of the filtrates of the incubation mixtures were determined in a test system using formalin-fixed platelets and Ristocetin as cofactor. The results so obtained indicate that thrombin inactivates the platelet-aggregating activity of factor VIII. The filtrates of the incubation mixtures were also tested by a two- stage test system for their clotting activities. Comparison of the influence of thrombin on the clotting- and the platelet-aggregating activities of factor VIII is presented and discussed.

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References

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