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CC BY-NC-ND 4.0 · International Journal of Epilepsy 2018; 05(02): 087-091
DOI: 10.1055/s-0038-1676907
Original Article
Indian Epilepsy Society

Possible Role of Heat Shock Protein 70 in Childhood Seizures

Magdy Mostafa Kamel
1   Department of Pediatrics, Faculty of Medicine, Minia University, Minia, Egypt
,
Samir Mohammed Mounir
1   Department of Pediatrics, Faculty of Medicine, Minia University, Minia, Egypt
,
Nagwa Ismail Okaily
2   Department of Clinical Pathology, Faculty of Medicine, Minia University, Minia, Egypt
,
Mohammed Hosny Abdelzaher
1   Department of Pediatrics, Faculty of Medicine, Minia University, Minia, Egypt
,
Mohammed Hosny Hassan
3   Department of Medical Biochemistry, Faculty of Medicine, South Valley University, Qena, Egypt
› Institutsangaben
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Publikationsverlauf

Received: 25. Oktober 2018

Accepted after revision: 14. November 2018

Publikationsdatum:
09. Januar 2019 (online)

   Lizenzen und Reprints

Abstract

Background There has been a long interest in investigating the relationship between heat shock protein (HSP) expression and the evidence of neuronal damage in the most susceptible brain areas after seizures. So, the present study aimed to assess heat shock protein (HSP70) in children with seizures (febrile seizures and epilepsy), and to find out the cutoff point of this marker that may help in confirming epilepsy diagnosis. The present study has been conducted to evaluate serum levels of HSP70 in children with epileptic and febrile seizures and to compare these results to that of healthy children.

Materials and Methods A prospective study included 85 children (32 females and 53 males) in Children and Maternity Unit, Minia University Hospital, Minia, Egypt. Children were subdivided into three groups, group (I) included 30 children with epilepsy, group (II) included 30 children with febrile seizures, and group (III) included 25 healthy children that served as a control group. HSP70 assay was performed for all included children using the enzyme-linked immunosorbent assay technique.

Results The overall results revealed significant high serum HSP70 levels in epilepsy and febrile seizures groups when compared with control group (p < 0.001). Also, HSP70 serum levels were significantly higher in epilepsy group than in febrile seizures group (p < 0.001). Serum HSP70 level at a cutoff point > 170 ng/L showed 60% sensitivity and specificity equal to 83.3% in prediction of epilepsy.

Conclusion HSP70 level was significantly higher in epileptic and febrile seizures children than normal healthy children, and HSP70 may be beneficial in confirming the diagnosis of epilepsy.

Keywords

children - epilepsy - febrile seizures - HSP70
 

  • References

  • 1 Wagner RG, Ngugi AK, Twine R. et al. Prevalence and risk factors for active convulsive epilepsy in rural northeast South Africa. Epilepsy Res 2014; 108 (04) 782-791
    CrossrefPubMedGoogle Scholar
  • 2 Saad K. Childhood epilepsy: an update on diagnosis and management. Am J Neurosci 2014; 5: 36
    PubMedGoogle Scholar
  • 3 Mikati M, Hani A. Seizures in childhood. In: Kliegman RM, Stanton BF, St Geme JW, Schor NF. eds. Nelson Textbook of Pediatrics. 20th ed.. Philadelphia, PA: Elsevier; 2016: 339-372
    Google Scholar
  • 4 Mahalka A, Kirkegaard T, Jukola L, Jäättelä M, Kinnunen P. Human heat shock protein 70 (Hsp70) as a peripheral membrane protein. Biochim Biophys Acta 2014; 1838: 1344-1361
    PubMedGoogle Scholar
  • 5 Yang T, Hsu C, Liao W, Chuang JS. Heat shock protein 70 expression in epilepsy suggests stress rather than protection. Acta Neuropathol 2008; 115 (02) 219-230
    CrossrefPubMedGoogle Scholar
  • 6 Cross JH, Kluger G, Lagae L. Advancing the management of childhood epilepsies. Eur J Paediatr Neurol 2013; 17 (04) 334-347
    CrossrefPubMedGoogle Scholar
  • 7 van Esch A, Steyerberg EW, Berger MY, Offringa M, Derksen-Lubsen G, Habbema JD. Family history and recurrence of febrile seizures. Arch Dis Child 1994; 70 (05) 395-399
    CrossrefPubMedGoogle Scholar
  • 8 Sfaihi L, Maaloul I, Kmiha S. et al. Febrile seizures: an epidemiological and outcome study of 482 cases. Childs Nerv Syst 2012; 28 (10) 1779-1784
    CrossrefPubMedGoogle Scholar
  • 9 Chung S, Ahn C. Effects of anti-epileptic drug therapy on bone mineral density in ambulatory epileptic children. Brain Dev 1994; 16 (05) 382-385
    CrossrefPubMedGoogle Scholar
  • 10 Hamed SA, Abdellah MM, El-Melegy N. Blood levels of trace elements, electrolytes, and oxidative stress/antioxidant systems in epileptic patients. J Pharmacol Sci 2004; 96 (04) 465-473
    CrossrefPubMedGoogle Scholar
  • 11 Gaitatzis A, Sander JW. The long-term safety of antiepileptic drugs. CNS Drugs 2013; 27 (06) 435-455
    CrossrefPubMedGoogle Scholar
  • 12 Gehrmann M, Marienhagen J, Eichholtz-Wirth H. et al. Dual function of membrane-bound heat shock protein 70 (Hsp70), Bag-4, and Hsp40: protection against radiation-induced effects and target structure for natural killer cells. Cell Death Differ 2005; 12 (01) 38-51
    CrossrefPubMedGoogle Scholar
  • 13 Pockley AG, Henderson B, Multhoff G. Extracellular cell stress proteins as biomarkers of human disease. Biochem Soc Trans 2014; 42 (06) 1744-1751
    CrossrefPubMedGoogle Scholar
  • 14 Lindquist S, Craig EA. The heat-shock proteins. Annu Rev Genet 1988; 22: 631-677
    CrossrefPubMedGoogle Scholar
  • 15 Ekimova IV, Nitsinskaya LE, Romanova IV, Pastukhov YF, Margulis BA, Guzhova IV. Exogenous protein Hsp70/Hsc70 can penetrate into brain structures and attenuate the severity of chemically-induced seizures. J Neurochem 2010; 115 (04) 1035-1044
    CrossrefPubMedGoogle Scholar
  • 16 Chang CC, Lui CC, Lee CC. et al. Clinical significance of serological biomarkers and neuropsychological performances in patients with temporal lobe epilepsy. BMC Neurol 2012; 12: 15
    CrossrefPubMedGoogle Scholar
  • 17 Kilic M, Gündüzalp M, Taskin E, Aydin S, Serin HM. Assessment of the levels of serum Hsp 70 and ghrelin in children with simple febrile convulsions. Minerva Pediatr 2016; 68 (02) 127-133
    PubMedGoogle Scholar
  • 18 Oraby AM, Raouf ER, El-Saied MM, Abou-Khadra MK, Helal SI, Hashish AF. Cognitive function and heat shock protein 70 in children with temporal lobe epilepsy. J Child Neurol 2017; 32 (01) 41-45
    CrossrefPubMedGoogle Scholar
  • 19 Turturici G, Sconzo G, Geraci F. Hsp70 and its molecular role in nervous system diseases. Biochem Res Int 2011; 2011: 618127
    PubMedGoogle Scholar
  • 20 Lu X, Wang X. Hyponatremia induced by antiepileptic drugs in patients with epilepsy. Expert Opin Drug Saf 2017; 16 (01) 77-87
    CrossrefPubMedGoogle Scholar
  • 21 Rejdak K, Kuhle J, Rüegg S. et al. Neurofilament heavy chain and heat shock protein 70 as markers of seizure-related brain injury. Epilepsia 2012; 53 (05) 922-927
    CrossrefPubMedGoogle Scholar