Facial plast Surg 2019; 35(01): 096-102
DOI: 10.1055/s-0039-1677709
Original Research
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

The Effect of Minocycline on Fat Graft Survival and Apoptotic Pathway

Kırdar Güney
1  Department of Plastic Surgery, René Clinic, Istanbul, Turkey
,
Sedat Tatar
2  Department of Plastic Reconstructive and Aesthetic Surgery, Koc Universitesi Tip Fakultesi, Istanbul, Turkey
,
Bora Özel
3  Department of Plastic Surgery, Memorial Hospital, Istanbul, Turkey
,
Cemile Seymen
4  Department of Histology and Embryology, Gazi Universitesi Tip Fakultesi, Ankara, Turkey
,
Çiğdem Elmas
4  Department of Histology and Embryology, Gazi Universitesi Tip Fakultesi, Ankara, Turkey
,
Serhan Tuncer
5  Department of Plastic Surgery, Gazi Universitesi Tip Fakultesi, Ankara, Turkey
,
Seyhan Çenetoğlu
5  Department of Plastic Surgery, Gazi Universitesi Tip Fakultesi, Ankara, Turkey
› Author Affiliations
Further Information

Publication History

Publication Date:
17 January 2019 (eFirst)

Abstract

Variable absorption rate is one of the biggest problems of fat grafting and one of the most important causes of fat graft volume loss is apoptosis. Minocycline is a tetracycline derivative and besides its antibacterial capacity, it has been widely using for anti-apoptotic effects. This study was designed to investigate the effect of minocycline on fat graft survival and adipocyte apoptosis. A total of two main and eight subgroups were designed and a total of 48 experimental animals, 6 in each group, were used. Fat grafts are obtained from Wistar albino rats and implanted to dorsal area of rats. Local and systemic minocycline was applied in the study groups. On the 9th day, apoptotic cells were detected by the terminal deoxynucleotidyl transferase dUTP nick end labeling method and on the 90th day morphologic characteristics and viability of adipocytes were evaluated using histologic and immunohistochemical methods and statistically compared. This study revealed that the fat grafts were bigger, and they kept their structures better and they were more vascular in the minocycline groups and apoptosis was significantly lower in the minocycline groups. The authors demonstrated that minocycline increases fat graft survival and statistical improvement in apoptosis inhibition via using minocycline therapy has been shown.