Endoscopy 2019; 51(04): S109-S110
DOI: 10.1055/s-0039-1681492
ESGE Days 2019 oral presentations
Saturday, April 6, 2019 11:00 – 13:00: Esophagus diagnosis and ablation South Hall 1B
Georg Thieme Verlag KG Stuttgart · New York

EFFECTIVENESS OF IRREVERSIBLE ELETROPORATION USING NEWLY DEVELOPED ENDOSCOPIC ABLATIVE CATHETERS IN GASTROINTESTINAL TRACT: AN IN VIVO ANIMAL STUDY

HJ Jeon
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
ES Kim
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
SH Jang
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
SH Kim
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
JH Kim
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
SJ Choi
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
SH Kim
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
JM Lee
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
HS Choi
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
YT Jeen
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
B Keum
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
HS Lee
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
HJ Chun
1   Korea University Anam Hospital, Seoul, Korea, Republic of
,
CD Kim
1   Korea University Anam Hospital, Seoul, Korea, Republic of
› Author Affiliations
Further Information

Publication History

Publication Date:
18 March 2019 (online)

Also available at
 

Aims:

Irreversible Electroporation (IRE) is an ablation technique that induces apoptosis by applying an electric field. IRE has several advantages over other ablation techniques. For this reason, the IRE has already been used as an adjunctive treatment in many areas of tumor therapy and has confirmed its possibility. The purpose of this study was to investigate the possibility of applying IRE therapy to gastrointestinal tract using newly designed endoscopic ablative catheters.

Methods:

The IRE was performed in the esophagus, stomach, and duodenum to six general anesthetized pigs. Two types of endoscopic IRE catheters were used. The gap between the electrodes was about 1.0 cm. One, A basket-shaped catheter was used for the esophagus and duodenum. The other is two electrode catheter used for stomach. The voltage was applied from 500V to 2000V, and the number of pulses was fixed to 60. The pigs were euthanized after 24hr from experiment day. After the IRE ablation on tissue, histological evaluation of ablation site was performed through H & E staining of the tissues.

Results:

Endoscopic IRE in the esophagus, stomach, and duodenum confirmed cell necrosis around the stimulation site. No damage was done to 500V in all tissues. The stomach caused necrosis of mucosa at 1000V, inflammation of submucosa at 1500V, and inflammation and vessel injury of all submucosa at 2000V. In the esophagus, seperation of the layer was observed at 1000V, desquamation of layer and erosion of mucosa were seen at 1500V, and inflammation of submucosa was induced at 2000V. In duodenum, erosion and necrosis were seen in mucosa at 500V, inflammation in submucosa at 1000V, and perforation at 2000V.

Conclusions:

Our newly designed catheters can be used to effectively ablate the esophagus, stomach, and duodenum. Further studies will be needed for the protocol about IRE ablation suited for esophagus, stomach and duodenum.