CC BY-NC-ND 4.0 · Laryngorhinootologie 2019; 98(S 02): S260
DOI: 10.1055/s-0039-1685991
Poster
Oncology

Differences in absolute numbers of leukocyte subsets in peripheral blood of head and neck cancer patients and healthy controls

C Gaede
1   HNO-Universitätsklinik Leipzig, Leipzig
,
S Melzer
2   Inst. f. Med. Informatik, Statistik u. Epidemiologie (IMISE) Leipzig, MedFak Leipzig, Leipzig
,
M Löffler
2   Inst. f. Med. Informatik, Statistik u. Epidemiologie (IMISE) Leipzig, MedFak Leipzig, Leipzig
,
A Dietz
1   HNO-Universitätsklinik Leipzig, Leipzig
,
JR Wenning
1   HNO-Universitätsklinik Leipzig, Leipzig
,
M Rosolowski
2   Inst. f. Med. Informatik, Statistik u. Epidemiologie (IMISE) Leipzig, MedFak Leipzig, Leipzig
,
A Tárnok
3   Translationszentrum für Regenerative Medizin (TRM), MedFak Leipzig, Leipzig
,
G Wichmann
1   HNO-Universitätsklinik Leipzig, Leipzig
› Author AffiliationsDie Studie wurde gefördert durch die Projekte LIFE-006 B7 und LIFE-007 D9 des Leipziger Forschungszentrums für Zivilisationserkrankungen (LIFE) der Universität Leipzig. LIFE wird gefördert durch die Europäische Union, den Europäischen Regional-Entwicklungsfond (EFRE), und den Freistaat Sachsen. The study was supported by the grants LIFE-006 B7 and LIFE-007 D9 of the Leipzig Research Center for Civilization Diseases (LIFE), University Leipzig. LIFE is funded by the European Union, the European Fund for Regional Development (EFRE), and the Free State of Saxony.
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Introduction:

The study's goal was to discover differences in leukocyte subsets between head and neck cancer patients (HNC-P) and healthy adults (HA) from a population-based cohort.

Material and Methods:

10-color flow cytometry (FCM) was used for in-depth immunophenotyping of peripheral blood (PB) samples of 608 HA and 101 therapy-naïve HNC-P. Absolute cell count (AbsCC) of leukocyte (CD45+) subsets were determined.

Results:

Comparing 608 HA and 101 HNC-P, differences (p < 0.01) in AbsCC were detected in granulocytes (side scatter high, SSChigh), neutrophiles (SSChighCD16+), monocytes (SSCmedCD14high), classical monocytes (SSCmedCD14highCD16dim), lymphocytes (SSClow), T-lymphocytes (CD3+CD16-CD56-), and T-cell subpopulations (CD3+CD8+ cytotoxic T-cells, CD3+CD4+ T-helper cells, and CD3+CD4+CD8+ double-positives).

Conclusion:

AbsCC allow for discrimination of HA and HNC-P but, however, had low power to discriminate HNC-P and HA. Consequently, we are aiming on analyses of relative cell counts to facilitate this approach.



Publication History

Publication Date:
23 April 2019 (online)

© 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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