Abstract
Objective The aim of this study was to evaluate the viability of stem cells from exfoliated
and deciduous teeth (SHED) on dentin surface treated with triple antibiotic paste
or calcium hydroxide.
Materials and Methods Nine single-rooted extracted premolars were prepared appropriately and divided into
three groups. In group A, the root canals were left empty, a triple antibiotic paste
was placed in the root canals of group B, and calcium hydroxide was placed in the
root canals of group C. After 1 week, the intracanal medicaments were removed, and
stem cells were seeded on the treated surface of the specimens for 1 more week. The
cells were stained and then observed under confocal microscope over the entire surface
of each test material. Counting of the cells was made by Image J (3D) software, as
well as manually.
Statistical Analysis To investigate any statistically significant differences between the experimental
groups, statistical tests including Kruskal–Wallis and Mann–Whitney U-test were performed.
Significance level was set to P < 0.05, and all analyses were performed with SPSS IBM program, v. 21.
Results Groups B and C showed statistically significantly higher number of cells compared
to Group A, whereas cells developed in a substrate of calcium hydroxide residues appeared
in majority with distinct cores and widened unlike other groups.
Conclusions The effect of calcium hydroxide manifested better results regarding the number of
stems cells on root canal surfaces.
Keywords
calcium hydroxide - regenerative endodontics - stem cells - triple antibiotic paste
