 
         
         ABSTRACT
         
         Objectives: The purpose of this in vitro study was to evaluate the effect of artificial
            saliva
            contamination on microtensile bond strength to pulp chamber dentin.
         
         Methods: Clearfil SE Bond (SEB) (Kuraray, JAPAN) adhesive system and Clearfil Photo
            Posterior (CPP) (Kuraray, JAPAN) composite resin were used. Twenty extracted caries-free
            human molar teeth were randomly distributed into four groups. Apart from a control
            group without contamination (Group 1), primed dentin surfaces were contaminated with
            artificial saliva (10 s), rinsed, dried, reprimed and bonded (Group 2), coated with
            adhesive, contaminated with artificial saliva, rinsed, dried, bonding procedures were
            repeated (Group 3), coated with adhesive, light cured, contaminated with saliva, rinsed,
            dried, treated with SE primer (SEP) and SEB (Group 4).
 
         
         After 24 hrs, the teeth were prepared for microtensile bond testing and tensile bond
            strength was measured (1mm/min). The data was calculated as MPa and analyzed using
            one-way ANOVA and Duncan test (P<.05).
         
         Results: The results indicated that Group 2 showed lowest bond strength when compared
            to the others (P<.05). No statistically significant difference was found between Groups
            3 and 4 (P>.05).
         
         Conclusions: It was concluded that contamination during priming procedure has a negative
            effect
            on bond strength (P<.05). Although contamination of the uncured adhesive was not critical
            in this study (P>.05) any kind of contamination of the bonding area should, in principle,
            be avoided. (Eur J Dent 2008;2:86-90)
         
         Key words:
Saliva contamination - Self-etch adhesive - Bond strength