Effectiveness of Ozone with or without the Additional Use of Remineralizing Solution on Non-Cavitated Fissure Carious Lesions in Permanent Molars

Didem Atabek; Nurhan Oztas

doi:10.1055/s-0039-1698911

European Journal of Dentistry, Table of Contents

CC BY-NC-ND 4.0 · Eur J Dent 2011; 05(04): 393-399
DOI: 10.1055/s-0039-1698911

Original Article

Dental Investigation Society

Effectiveness of Ozone with or without the Additional Use of Remineralizing Solution on Non-Cavitated Fissure Carious Lesions in Permanent Molars

Authors

Didem Atabek

^aGazi University, Faculty of Dentistry, Department of Pediatric Dentistry, Ankara, Turkey.
Nurhan Oztas

^aGazi University, Faculty of Dentistry, Department of Pediatric Dentistry, Ankara, Turkey.

Abstract

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ABSTRACT

Objectives: The aim of this study was to evaluate the efficiency of ozone alone and with a re-mineralizing solution following application on initial pit and fissure caries lesions in permanent molars. Methods: Forty children (9-12 years) having non-cavitated fissure caries lesions on bilateral 40 first permanent mandibular molar teeth were participated in the study. Patients were randomly allocated to 2 experimental groups consisting of 20 subjects. In the first group, ozone was applied once for 40 seconds to the assigned test teeth of each pair. In the second group, ozone was applied once for 40 seconds to the assigned test teeth of each pair with the use of re-mineralizing solution. Progression or improvement of the caries was assessed at baseline, immediately after treatment and at 1-,2-,3-, and 6 month follow-up by comparing the DIAGNOdent values, Clinical Severity Indexes, Oral Hygiene Scores. The results were analyzed statistically by using the Wilcoxon-Test for dependent samples in each group. When comparing different test groups (control and experimental groups) the Friedman S test followed by the Mann-Whitney U test was used. Results: A statistically significant difference was found between all of the control and experimental test lesions in each group (P<.001). However, there was no statistically significant difference between the ozone treated groups and those using the additional re-mineralizing solution (P>.001). Conclusions: Ozone treatment either alone or combined with a re-mineralizing solution was found to be effective for remineralization of initial fissure caries lesions. (Eur J Dent 2011;5:393-399)

Key words:

Ozone - Fissure caries - Preventive dentistry

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References

REFERENCES
1 Müller P, Guggenheim B, Schmidlin PR. Efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. Eur J Oral Sci 2007;115:77-80.
2 Murdoch-Kinch CA. Minimal invasive dentistry. J Am Dent Assoc 2003;134:87-95.
3 Featherstone JD. The science and practice of caries prevention. J Am Dent Assoc 2000;131:887-899.
4 Baysan A, Lynch E. Effect of ozone on the oral microbiota and clinical severity of primary root caries. Am J Dent 2004;17:56-60.
5 Huth KC, Paschos E, Brand K, Hickel R. Effect of ozone on non-cavitated fissure carious lesions in permanent molars. A controlled prospective clinical study. Am J Dent 2005;18:223-228.
6 Nogales CG, Ferrari PH, Kantorovich EO, Lage-Marques JL. Ozone therapy in medicine and dentistry. J Contemp Dent Pract 2008;9:75-84.
7 Dähnhardt JE, Jaeggi T, Lussi A. Treating open carious lesions in anxious children with ozone. A prospective controlled clinical study. Am J Dent 2006;19:267-270.
8 Nagayoshi M, Kitamura C, Fukuizumi T, Nishihara T, Terashita M. Antimicrobial effect of ozonated water on bacteria invading dentinal tubules. J Endod 2004;30:778-781.
9 Oizumi M, Suzuki T, Uchida M, Furuya J, Okamoto Y. Invitro testing of a denture cleaning method using ozone. J Med Dent Sci 1998;45:135-139.
10 Baysan A, Beighton D. Assessment of the ozone-mediated killing of bacteria in infected dentine associated with noncavitated occlusal carious lesions. Caries Res 2007;41:337- 341.
11 Velano HE, do Nascimento LC, de Barros LM, Panzeri H. In vitro assessment of antibacterial activity of ozonized water against Staphylococcus aureus. Pesqui Odontol Bras 2001;15:18-22.
12 Baysan A, Whiley RA, Lynch E. Antimicrobial effect of a novel ozone- generating device on micro-organisms associated with primary root carious lesions in vitro. Caries Res 2000;34:498-501.
13 Lynch E. Antimicrobial management of primary root carious lesions: a review. Gerodontology 1996;13:118-129.
14 Ekstrand KR, Ricketts DNJ, Kidd EAM, Ovist V, Schou S. Detection, diagnosis, monitoring and logical treatment of occlusal caries in relation to lesion activity and severity: an in vivo examination with histological validation. Caries Res 1998;32:247-254.
15 Featherstone JD. Caries prevention and reversal based on the caries balance. Pediatr Dent 2006;28:128-132; discussion 192-198.
16 Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50:353-380.
17 ten Cate JM, Featherstone JD. Mechanistic aspects of the interactions between fluoride and dental enamel. Crit Rev Oral Biol Med 1991;2:283-296.
18 Lamkin MS, Oppenheim FG. Structural features of salivary function. Crit Rev Oral Biol Med 1993;4:251-259.
19 Lynch H, Milgrom P. Xylitol and dental caries: an overview for clinicians. J Calif Dent Assoc 2003;31:205-209.
20 Featherstone JD, Gansky SA, Hoover CI. A randomized clinical trial of caries management by risk assessment. Caries Res 2005;39:295.
21 Yamayoshi T, Tatsumi N. Microbicidal effects of ozone solution on methicillin-resistant Staphylococcus aureus. Drugs Exp Clin Res 1993;19:59-64.
22 Hems RS, Gulabivala K, Ng YL, Ready D, Spratt DA. An in vitro evaluation of the ability of ozone to kill a strain of Enterococcus faecalis. Int Endod J 2005;38:22-29.
23 Sechi LA, Lezcano I, Nunez N, Espim M, Duprè I, Pinna A, Molicotti P, Fadda G, Zanetti S. Antibacterial activity of ozonized sunflower oil (Oleozon). J Appl Microbiol 2001;90:279-284.
24 Cardoso MG, de Oliveira LD, Koga-Ito CY, Jorge AO. Effectiveness of ozonated water on Candida albicans, Enterococcus faecalis, and endotoxins in root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;105:e85-91.
25 Baysan A, Lynch E. The use of ozone in dentistry and medicine. Part 2. Ozone and root caries. Prim Dent Care 2006;13:37-41.
26 Baysan A, Lynch E, Grootveld M. The use of ozone for the management of primary root carious lesions. Tissue preservation and caries treatment. Quintessence Book 2001;3:49-67.
27 Hodson N, Dunne SM. Using ozone to treat dental caries. J Esthet Restor Dent 2007;19:303-305.
28 Kramer PF, Zelante F, Simionato MR. The immediate and long-term effects of invasive and noninvasive pit and fissure sealing techniques on the microflora in occlusal fissures of human teeth. Pediatr Dent 1993;15:108-112.
29 Theilade E, Fejerskov O, Migasena K, Prachyabrued W. Effect of fissure sealing on the microflora in occlusal fissures of human teeth. Arch Oral Biol 1977;22:251-259.
30 Hugoson A, Lundgren D, Asklöw B, Borgklint G. Effect of three different dental health preventive programmes on young adult individuals: a randomized, blinded, parallel group, controlled evaluation of oral hygiene behaviour on plaque and gingivitis. J Clin Periodontol 2007;34:407-415.
31 Tan HH, Ruiter E, Verhey H. Effects of repeated dental health care education on gingival health, knowledge, attitude, behavior and perception. Community Dent Oral Epidemiol 1981;9:15-21.