Abstract
This study revealed the reason behind the antibiotics resistance of isolated food-borne
pathogens through their susceptibility testing to various antibiotics of choice. The
results of the study revealed that resistance of the bacteria isolates which are Streptococcus
sp., Staphylococcus aureus, Proteus vulgaris, Shigella sp., Escherichia coli, Pseudomonas
aeruginosa, Bacillus sp. to different antibiotics varies and differs considerably.
For instance, Pseudomonas aeruginosa showed resistance to Amoxicillin, Augmentin,
Gentamicin and Tetracycline. Staphylococcus sp. isolated showed multiple resistances
to Cloxacillin, Erythromycin, Amoxicillin, Augmentin and Gentamicin. Proteus vulgaris
showed multiple resistances to five antibiotics in-vitro which are Augmentin, Nitrofurantoin,
Amoxicillin, Cotrimoxazole and Nalidixic. The seven isolates were then assayed for
plasmid profiling by agarose gel electrophoresis. All the isolates has plasmid with
varying sizes of between 9–21kb. Further conjugative study will reveal more reason
behind the resistance.
Keywords
Antibiotics - Resistance - Susceptibility - Plasmid