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DOI: 10.1055/s-0040-1711059
Characterization of chondrocytes and chondrogenic progenitor cells isolated from auricular cartilage and perichondrium of microtia patients and healthy donors
Introduction Isolated chondrocytes (CC) and chondrogenic progenitor cells (CPC) from microtia patients could be a useful source for autologous cartilage regeneration. However, little is known about cell biology and cell behaviour of CC and CPC isolated from the remnant auricular cartilage of microtia patients. Therefore, this study aimed to characterize these cells and to evaluate their capability to regenerate cartilage tissue for treating microtia.
Methods Cells were isolated from auricular cartilage and perichondrium of microtia patients (n = 3) and healthy donors (n = 3) using outgrowth cell culture and enzymatic digestion. (ethical approval nr.: 2018-584N-MA) To investigate general cell properties a colony forming assay and a migration assay were performed. In a 3D cell culture model, cells were cultured as spheroids (100.000 cells/spheroid). Spheroids were analysed for cartilage specific markers (e.g. collagen II, elastin, etc.) and specific progenitor cell markers like Integrin β1 or Notch-1 using immunological methods (Western blot and immunohistochemistry).
Results Regarding general cell properties, no significant difference was observed between cells from microtia patients or healthy tissue. CPCs showed a higher capacity to form colonies and to migrate than CCs. All cell types formed spheroids and expressed cartilaginous matrix. CPCs isolated from the perichondrium expressed higher amounts of Notch-1 and Integrin β1 in contrast to CPCs isolated from the cartilage.
Conclusions Our results showed that CCs and CPCs isolated from microtia patients possess a capacity for cartilage regeneration. Therefore, further studies should be performed to evaluate them as a useful source for autologous cartilage reconstruction.
Gefördert durch den Schweizer Nationalfond (Sinergia-Projekt: 2-77120-17)
Poster-PDF A-1267.PDF
Publication History
Article published online:
10 June 2020
© 2020. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
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