CC BY-NC-ND 4.0 · Laryngorhinootologie 2020; 99(S 02): S400-S401
DOI: 10.1055/s-0040-1711446
Abstracts
Tissue Engineering / Stem Cells

The Response of Primary Human Macrophages to Decellularized Cartilage Extracellular Matrix

D Gvaramia
1   Medical Faculty Mannheim, Ruprecht-Karls-University Heidelberg, Department of Otorhinolaryngology, Head and Neck Surgery, Mannheim
,
J Kern
1   Medical Faculty Mannheim, Ruprecht-Karls-University Heidelberg, Department of Otorhinolaryngology, Head and Neck Surgery, Mannheim
,
Y Jakob
1   Medical Faculty Mannheim, Ruprecht-Karls-University Heidelberg, Department of Otorhinolaryngology, Head and Neck Surgery, Mannheim
,
L Huber
2   University Medical Center Mannheim, Department of Otorhinolaryngology, Head and Neck Surgery, Mannheim
,
N Rotter
1   Medical Faculty Mannheim, Ruprecht-Karls-University Heidelberg, Department of Otorhinolaryngology, Head and Neck Surgery, Mannheim
› Author Affiliations
 

Introduction Extracellular matrix (ECM)-based biomaterials found common use in tissue engineering due to their cell-instructive properties and biodegradability. However, the host immune response can be a determining factor for successful regeneration after biomaterial transplantation. Macrophages are responsible not only for the inflammatory reaction by M1 but also for the process of constructive remodeling of biomaterials by M2 macrophages.

Methods Here we used an in vitro approach to evaluate polarization of primary human monocyte-derived macrophages (MDM) into M1 or M2 macrophages in response to the porcine nasal decellularized cartilage extracellular matrix (DECM). Porcine ECM-based materials – small intestinal submucosa (SIS) and Parietex™ Composite were used as a reference. As a positive control, cells were treated with IFN γ or IL-4 to induce M1 or M2 phenotype, respectively. Tissue culture polystyrene (TCPS) with no further treatment was used as a negative control. MDM were analyzed for the expression of characteristic surface markers, as well as gene expression and secretion of inflammatory cytokines.

Results Initially, higher gene expression and protein levels of pro-inflammatory cytokines, TNF-a and IL-1ß were detected in the DECM culture compared to the controls. However, the secretion of these factors was later replaced by the production of the remodeling cytokine, CCL18. The expression of the pro-inflammatory surface marker, CD38 was generally low in all conditions, being highest on TCPS and SIS. In contrast, the highest increase of the M2-specific CD206 was seen on DECM as compared to the controls.

Conclusion Overall, while DECM induced MDM differentiation, the persistence of inflammatory reaction was not observed. Rather polarization towards M2 was detected.

Poster-PDF A-1574.PDF



Publication History

Article published online:
10 June 2020

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