CC BY-NC-ND 4.0 · Laryngorhinootologie 2020; 99(S 02): S402
DOI: 10.1055/s-0040-1711450
Abstracts
Tissue Engineering / Stem Cells

Biphasic electrical stimulation promotes proliferation and up-regulate brain-derived neurotrophic factor expression in rat olfactory bulb neural precursor cells

M Wang
1   Peking University International Hospital, Otolaryngolgy, Peking China
› Author Affiliations
 

Transplanted olfactory bulb neural precursor cells (OB NPCs) into the damaged inner ear may be considered as a valuable remedy exploration for hearing regeneration. However, the therapeutic prospects are limited by multiple factors, such as immune reaction, oxidative stress, limited blood supply and trophic factor deficiency. Electrical stimulation (ES) has recently been found to participate in the regulation of cell proliferation,growth, differentiation and migration, but its underlying effects on OB NPCs remain unclear. In this study, we investigate the proliferative capacity, morphologic alterations of OB NPCs that occur when exposed 6 hours to biphasic electrical stimulation (BES) of 100 mV/mm and 200 mV/mm electric field strengths with a pulse-burst pattern and 8 ms pulses (20 % duty cycle). Cells that were not exposed to BES served as controls. For both BES treatments, the MTT results revealed that the cellular proliferation of exposed group during the last 6 h of the experiment were statistically higher than control. Then, we investigated morphological structure changes in the cells assessed by TEM. Compare with control group, most of cells were present at intensively proliferating appearance included the microfilaments were long and thick and the accumulated appearance of cells. In addition, BDNF secretion increased with BES for 12 h. It is conceivable that electrical fields as a new approach may promote the growth and proliferation of NPCs and may be engineered to control the survival of transplanted NPCs in injured inner ear and acoustic nerve. Although our results have been suggesting that EFs may be non-chemical strategies for cell proliferation, the fundamental mechanisms remained to be elucidated.

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Supported by This study was supported by the National Natural Science Foundation of China (11202018), the Post-doctoral Foundation of China (20110490269, 2013T60055) and Peking University International Hospital Research Fund (YN2016QN03).



Publication History

Article published online:
10 June 2020

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