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CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(04): 575-583
DOI: 10.1055/s-0040-1713956
Original Article

Effect of Hydrogel-Based Antibiotic Intracanal Medicaments on Push-Out Bond Strength

Rayan B. Yaghmoor
1   Department of Restorative Dentistry, College of Dental Medicine, Umm Al-Qura university, Makkah, Saudi Arabia
2   Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, Indianapolis, Indiana, United States
,
Jeffrey A. Platt
2   Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, Indianapolis, Indiana, United States
,
Kenneth J. Spolnik
3   Department of Endodontics, Indiana University School of Dentistry, Indianapolis, Indiana, United States
,
Tien Min Gabriel Chu
2   Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, Indianapolis, Indiana, United States
,
Ghaeth H. Yassen
2   Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, Indianapolis, Indiana, United States
4   Department of Endodontics, Case School of Dental Medicine, Cleveland, Ohio, United States
› Author Affiliations Funding This study received its financial support from Delta Dental Foundation.
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Abstract

Objective This study aimed to evaluate the effects of typical clinical concentration (1,000 mg/mL), low concentration (1 mg/mL) triple antibiotic pastes (TAP), and double antibiotic pastes (DAP) on the bond strength between various root cements and radicular dentin.

Materials and Methods Intact single-rooted human teeth (n = 144) were horizontally decoronated and canals instrumented. The roots were treated for 4 weeks with Ca(OH)2, 1,000 mg/mL of TAP or DAP, and 1 mg/mL of TAP or DAP. Untreated roots served as a control. After treatment, the medicaments were irrigated and each group was divided into three subgroups receiving MTA, Biodentine, or Endosequence putty cement. After 2 weeks, coronal and middle root cylinders were obtained from each root. Push-out bond strength test and failure analysis were performed for all root cylinders.

Statistical Analysis  Three-way ANOVA, pairwise comparisons and logistic regression were used for statistical analyses. A significance level of 5% was used.

Results  For MTA applied in the coronal part of the roots, 1 mg/mL DAP and TAP and Ca(OH)2 demonstrated significantly higher bond strength compared with the typical clinical concentration and the control groups. For Biodentine applied coronally in the roots, 1 mg/mL of DAP resulted in significantly higher bond strength than all other groups. For Endosequence putty cement applied coronally in the roots, 1 mg/mL of DAP offered significantly higher bond strength than all groups except for Ca(OH)2.

Conclusion The use of 1 mg/mL DAP resulted in significantly higher push-out bond strength compared with the typical clinical concentration of TAP and DAP regardless of the type of the root cement used.

Keywords

bond strength - intracanal medicaments - regenerative endodontics


Publication History

Article published online:
29 July 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
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  • References

  • 1 Ding RY, Cheung GS, Chen J, Yin XZ, Wang QQ, Zhang CF. Pulp revascularization of immature teeth with apical periodontitis: a clinical study. J Endod 2009; 35 (05) 745-749
    CrossrefPubMedGoogle Scholar
  • 2 Wigler R, Kaufman AY, Lin S, Steinbock N, Hazan-Molina H, Torneck CD. Revascularization: a treatment for permanent teeth with necrotic pulp and incomplete root development. J Endod 2013; 39 (03) 319-326
    CrossrefPubMedGoogle Scholar
  • 3 Chen MY, Chen KL, Chen CA, Tayebaty F, Rosenberg PA, Lin LM. Responses of immature permanent teeth with infected necrotic pulp tissue and apical periodontitis/abscess to revascularization procedures. Int Endod J 2012; 45 (03) 294-305
    CrossrefPubMedGoogle Scholar
  • 4 Lovelace TW, Henry MA, Hargreaves KM, Diogenes A. Evaluation of the delivery of mesenchymal stem cells into the root canal space of necrotic immature teeth after clinical regenerative endodontic procedure. J Endod 2011; 37 (02) 133-138
    CrossrefPubMedGoogle Scholar
  • 5 Trope M. Treatment of the immature tooth with a non-vital pulp and apical periodontitis. Dent Clin North Am 2010; 54 (02) 313-324
    CrossrefPubMedGoogle Scholar
  • 6 AAE. Clinical considerations for a regenerative procedures. American Association of Endodontists. Available at: https://www.aae.org/specialty/clinical-resources/regenerative-endodontics/. Published 2018. Updated January 4, 2018. Accessed October 13, 2019
  • 7 Ruparel NB, Teixeira FB, Ferraz CC, Diogenes A. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla. J Endod 2012; 38 (10) 1372-1375
    CrossrefPubMedGoogle Scholar
  • 8 Yassen GH, Eckert GJ, Platt JA. Effect of intracanal medicaments used in endodontic regeneration procedures on microhardness and chemical structure of dentin. Restor Dent Endod 2015; 40 (02) 104-112
    CrossrefPubMedGoogle Scholar
  • 9 Yassen GH, Vail MM, Chu TG, Platt JA. The effect of medicaments used in endodontic regeneration on root fracture and microhardness of radicular dentine. Int Endod J 2013; 46 (07) 688-695
    CrossrefPubMedGoogle Scholar
  • 10 Topçuoğlu HS, Arslan H, Akçay M, Saygili G, Çakici F, Topçuoğlu G. The effect of medicaments used in endodontic regeneration technique on the dislocation resistance of mineral trioxide aggregate to root canal dentin. J Endod 2014; 40 (12) 2041-2044
    CrossrefPubMedGoogle Scholar
  • 11 El-Ma’aita AM, Qualtrough AJ, Watts DC. The effect of smear layer on the push-out bond strength of root canal calcium silicate cements. Dent Mater 2013; 29 (07) 797-803
    CrossrefPubMedGoogle Scholar
  • 12 Turk T, Ozisik B, Aydin B. Time-dependent effectiveness of the intracanal medicaments used for pulp revascularization on the dislocation resistance of MTA. BMC Oral Health 2015; 15 (01) 130
    CrossrefPubMedGoogle Scholar
  • 13 Jacobs JC, Troxel A, Ehrlich Y. et al. Antibacterial effects of antimicrobials used in regenerative endodontics against biofilm bacteria obtained from mature and immature teeth with necrotic pulps. J Endod 2017; 43 (04) 575-579
    CrossrefPubMedGoogle Scholar
  • 14 McIntyre PW, Wu JL, Kolte R. et al. The antimicrobial properties, cytotoxicity, and differentiation potential of double antibiotic intracanal medicaments loaded into hydrogel system. Clin Oral Investig 2019; 23 (03) 1051-1059
    CrossrefPubMedGoogle Scholar
  • 15 Dawood AE, Parashos P, Wong RHK, Reynolds EC, Manton DJ. Calcium silicate-based cements: composition, properties, and clinical applications. J Investig Clin Dent 2017;8(2)
  • 16 Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review–part II: leakage and biocompatibility investigations. J Endod 2010; 36 (02) 190-202
    CrossrefPubMedGoogle Scholar
  • 17 Prather BT, Ehrlich Y, Spolnik K, Platt JA, Yassen GH. Effects of two combinations of triple antibiotic paste used in endodontic regeneration on root microhardness and chemical structure of radicular dentine. J Oral Sci 2014; 56 (04) 245-251
    CrossrefPubMedGoogle Scholar
  • 18 Yassen GH, Huang R, Al-Zain A, Yoshida T, Gregory RL, Platt JA. Evaluation of selected properties of a new root repair cement containing surface pre-reacted glass ionomer fillers. Clin Oral Investig 2016; 20 (08) 2139-2148
    CrossrefPubMedGoogle Scholar
  • 19 Yassen GH, Sabrah AH, Eckert GJ, Platt JA. Effect of different endodontic regeneration protocols on wettability, roughness, and chemical composition of surface dentin. J Endod 2015; 41 (06) 956-960
    CrossrefPubMedGoogle Scholar
  • 20 Nerness AZ, Ehrlich Y, Spolnik K, Platt JA, Yassen GH. Effect of triple antibiotic paste with or without ethylenediaminetetraacetic acid on surface loss and surface roughness of radicular dentine. Odontology 2016; 104 (02) 170-175
    CrossrefPubMedGoogle Scholar
  • 21 Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod 2005; 31 (02) 97-100
    CrossrefPubMedGoogle Scholar
  • 22 Reyes-Carmona JF, Felippe MS, Felippe WT. The biomineralization ability of mineral trioxide aggregate and Portland cement on dentin enhances the push-out strength. J Endod 2010; 36 (02) 286-291
    CrossrefPubMedGoogle Scholar
  • 23 Minabe M, Takeuchi K, Kumada H, Umemoto T. The effect of root conditioning with minocycline HCl in removing endotoxin from the roots of periodontally-involved teeth. J Periodontol 1994; 65 (05) 387-392
    CrossrefPubMedGoogle Scholar
  • 24 Shokouhinejad N, Yazdi KA, Nekoofar MH, Matmir S, Khoshkhounejad M. Effect of acidic environment on dislocation resistance of endosequence root repair material and mineral trioxide aggregate. J Dent (Tehran) 2014; 11 (02) 161-166
    Google Scholar
  • 25 Guneser MB, Akbulut MB, Eldeniz AU. Effect of various endodontic irrigants on the push-out bond strength of biodentine and conventional root perforation repair materials. J Endod 2013; 39 (03) 380-384
    CrossrefPubMedGoogle Scholar
  • 26 Lucas CP, Viapiana R, Bosso-Martelo R, Guerreiro-Tanomaru JM, Camilleri J, Tanomaru-Filho M. Physicochemical properties and dentin bond strength of a tricalcium silicate-based retrograde material. Braz Dent J 2017; 28 (01) 51-56
    CrossrefPubMedGoogle Scholar
  • 27 Akcay H, Arslan H, Akcay M, Mese M, Sahin NN. Evaluation of the bond strength of root-end placed mineral trioxide aggregate and Biodentine in the absence/presence of blood contamination. Eur J Dent 2016; 10 (03) 370-375
    Article in Thieme ConnectPubMedGoogle Scholar
  • 28 Nagas E, Cehreli ZC, Uyanik MO, Vallittu PK, Lassila LV. Effect of several intracanal medicaments on the push-out bond strength of ProRoot MTA and Biodentine. Int Endod J 2016; 49 (02) 184-188
    CrossrefPubMedGoogle Scholar
  • 29 Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. Int Endod J 2011; 44 (12) 1081-1087
    CrossrefPubMedGoogle Scholar
  • 30 Camilleri J, Sorrentino F, Damidot D. Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus. Dent Mater 2013; 29 (05) 580-593
    CrossrefPubMedGoogle Scholar
  • 31 Aydin MN, Buldur B. The effect of intracanal placement of various medicaments on the bond strength of three calcium silicate-based cements to root canal dentin. J Adhes Sci Technol 2017; 32 (05) 542-552
    CrossrefGoogle Scholar
  • 32 Patil U, Yeli M, Tapashetti S, Naik B, Tilakchand M. Effect of varying durations of intracanal medicament application used in regenerative endodontic treatment on the push-out bond strength of a novel cement: NeoMTA Plus. J Conserv Dent 2019; 22 (01) 48-53
    PubMedGoogle Scholar
  • 33 Amin SAW, Gawdat SI. Retention of BioAggregate and MTA as coronal plugs after intracanal medication for regenerative endodontic procedures: an ex vivo study. Restor Dent Endod 2018; 43 (03) e18
    CrossrefPubMedGoogle Scholar
  • 34 Oktay EA, Ersahan S, Gokyay S. Effect of intracanal medicaments used in endodontic regeneration on the push-out bond strength of a calcium-phosphate-silicate-based cement to dentin. Pak J Med Sci 2018; 34 (02) 310-315
    CrossrefPubMedGoogle Scholar
  • 35 Gokturk H, Bayram E, Bayram HM, Aslan T, Ustun Y. Effect of double antibiotic and calcium hydroxide pastes on dislodgement resistance of an epoxy resin-based and two calcium silicate-based root canal sealers. Clin Oral Investig 2017; 21 (04) 1277-1282
    CrossrefPubMedGoogle Scholar
  • 36 Bidar M, Disfani R, Gharagozloo S, Khoynezhad S, Rouhani A. Medication with calcium hydroxide improved marginal adaptation of mineral trioxide aggregate apical barrier. J Endod 2010; 36 (10) 1679-1682
    CrossrefPubMedGoogle Scholar
  • 37 Collares FM, Portella FF, Rodrigues SB, Celeste RK, Leitune VCB, Samuel SMW. The influence of methodological variables on the push-out resistance to dislodgement of root filling materials: a meta-regression analysis. Int Endod J 2016; 49 (09) 836-849
    CrossrefPubMedGoogle Scholar
  • 38 Weis MV, Parashos P, Messer HH. Effect of obturation technique on sealer cement thickness and dentinal tubule penetration. Int Endod J 2004; 37 (10) 653-663
    CrossrefPubMedGoogle Scholar
  • 39 Mannocci F, Pilecki P, Bertelli E, Watson TF. Density of dentinal tubules affects the tensile strength of root dentin. Dent Mater 2004; 20 (03) 293-296
    CrossrefPubMedGoogle Scholar
  • 40 Ferrari M, Mannocci F, Vichi A, Cagidiaco MC, Mjör IA. Bonding to root canal: structural characteristics of the substrate. Am J Dent 2000; 13 (05) 255-260
    PubMedGoogle Scholar
  • 41 Paqué F, Luder HU, Sener B, Zehnder M. Tubular sclerosis rather than the smear layer impedes dye penetration into the dentine of endodontically instrumented root canals. Int Endod J 2006; 39 (01) 18-25
    CrossrefPubMedGoogle Scholar
  • 42 Marion D, Jean A, Hamel H, Kerebel LM, Kerebel B. Scanning electron microscopic study of odontoblasts and circumpulpal dentin in a human tooth. Oral Surg Oral Med Oral Pathol 1991; 72 (04) 473-478
    CrossrefPubMedGoogle Scholar
  • 43 Alsubait S, Alsaad N, Alahmari S, Alfaraj F, Alfawaz H, Alqedairi A. The effect of intracanal medicaments used in Endodontics on the dislocation resistance of two calcium silicate-based filling materials. BMC Oral Health 2020; 20 (01) 57
    CrossrefPubMedGoogle Scholar