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CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(04): 598-604
DOI: 10.1055/s-0040-1714172
Original Article

Evaluating the Abrasivity of a Nanosized Eggshell–Titanium Dioxide on Tooth Enamel Using Atomic Force Microscopy

Stanley Chibuzor Onwubu
1   Department of Dental Sciences, Durban University of Technology (DUT), Durban, South Africa
,
Phumlane Selby Mdluli
2   Department of Chemistry, Durban University of Technology (DUT), Durban, South Africa
,
Shenuka Singh
3   Discipline of Dentistry, University of KwaZulu-Natal (UKZN), Durban, South Africa
,
Sanele Nyembe
4   DST/Mintek Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Randburg, Johannesburg, South Africa
,
Rookmoney Thakur
5   Public Management and Economics, Durban University of Technology (DUT), Durban, South Africa
› Author Affiliations Funding This research was funded by the National Research Foundation of South Africa (No. 104824)
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Abstract

Objectives This study aimed to evaluate nanosized eggshell–titanium dioxide (EB@TiO2) abrasiveness in comparison with calcium carbonate and hydrated silica-containing toothpaste.

Material and Methods Thirty-five bovine tooth enamels were prepared, and specimens randomly assigned to a sample group of five (n = 7), namely, Colgate regular, Colgate pro-relief, Sensodyne rapid relief, Sensodyne repair, and EB@TiO2. Half of the enamel surface was brushed with each respective sample group, while the other half was covered with a tap.

Statistical Analysis The mean roughness value (Ra) of the brushed and covered halves were measured using an atomic force microscope (AFM). To assess the surface morphology and changes, a scanning electron microscope (SEM) was performed. Using pair sample test and ANOVA, the Ra for the entire specimens were analyzed. A Bonferroni correction was used to identify the mean differences among the five groups (α=.05).

Results  The findings from this analysis show that all the tested toothpaste abraded the enamel significantly (p < 0.05). The abrasive value contained in toothpaste comprising calcium carbonate was lower than the silica toothpaste hydrated.

Conclusion Overall, Colgate regular had the lowest toothpaste abrasivity, followed by EB@TiO2, while Sensodyne rapid relief had the most enamel wear. The prominent feature of this study suggests that EB@TiO2 is suitable for oral use, as its abrasivity is comparable with calcium carbonate-containing toothpaste.

Keywords

abrasivity - enamel - toothpaste - Atomic Force Microscope


Publication History

Article published online:
10 August 2020

© .

Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India

 

  • References

  • 1 Abhay S, Dinnimath B, Hullatti K. Formulation and spectral analysis of new polyherbal toothpaste. J Drug Deliv Ther 2014; 4 (06) 68-74
    Google Scholar
  • 2 Lippert F. An Introduction to Toothpaste-Its Purpose, History and Ingredients. Basel, Switzerland: Toothpastes Karger Publishers; 2013: 1-14
    Google Scholar
  • 3 Addy M, Hunter ML. Can tooth brushing damage your health? Effects on oral and dental tissues. Int Dent J 2003; 53 (Suppl. 03) 177-186
    CrossrefPubMedGoogle Scholar
  • 4 Arnold WH, Prange M, Naumova EA. Effectiveness of various toothpastes on dentine tubule occlusion. J Dent 2015; 43 (04) 440-449
    CrossrefPubMedGoogle Scholar
  • 5 Joiner A. Whitening toothpastes: a review of the literature. J Dent 2010; 38 (Suppl. 02) e17-e24
    CrossrefPubMedGoogle Scholar
  • 6 Hara AT, Turssi CP. Baking soda as an abrasive in toothpastes: Mechanism of action and safety and effectiveness considerations. J Am Dent Assoc 2017; 148 (11S) S27-S33
    CrossrefPubMedGoogle Scholar
  • 7 Giles A, Claydon NC, Addy M, Hughes N, Sufi F, West NX. Clinical in situ study investigating abrasive effects of two commercially available toothpastes. J Oral Rehabil 2009; 36 (07) 498-507
    CrossrefPubMedGoogle Scholar
  • 8 Field JC. The investigation of enamel subjected to early erosive and abrasive challenges. Newcastle: University; 2012
    Google Scholar
  • 9 Absi EG, Addy M, Adams D. Dentine hypersensitivity–the effect of toothbrushing and dietary compounds on dentine in vitro: an SEM study. J Oral Rehabil 1992; 19 (02) 101-110
    CrossrefPubMedGoogle Scholar
  • 10 Dyer D, Addy M, Newcombe RG. Studies in vitro of abrasion by different manual toothbrush heads and a standard toothpaste. J Clin Periodontol 2000; 27 (02) 99-103
    CrossrefPubMedGoogle Scholar
  • 11 Hara AT, Kelly SA, González-Cabezas C. et al. Influence of fluoride availability of dentifrices on eroded enamel remineralization in situ. Caries Res 2009; 43 (01) 57-63
    CrossrefPubMedGoogle Scholar
  • 12 Anusavice KJ, Antonson SA. Materials and processes for cutting, grinding, finishing, and polishing. In: Anusavice KJ, Shen C, Rawls HR. eds. Phillips’ Science of Dental Materials. St. Louis: Saunders; 2013: 236
    Google Scholar
  • 13 Bizhang M, Riemer K, Arnold WH, Domin J, Zimmer S. Influence of bristle stiffness of manual toothbrushes on eroded and sound human dentin-an in vitro study. PLoS One 2016; 11 (04) e0153250
    CrossrefPubMedGoogle Scholar
  • 14 Arnold WH, Gröger Ch, Bizhang M, Naumova EA. Dentin abrasivity of various desensitizing toothpastes. Head Face Med 2016; 12 (01) 16
    CrossrefPubMedGoogle Scholar
  • 15 Addy M. Tooth brushing, tooth wear and dentine hypersensitivity–are they associated?. Int Dent J 2005; 55 (04) (Suppl. 01) 261-267
    CrossrefPubMedGoogle Scholar
  • 16 Onwubu SC, Vahed A, Singh S, Kanny KM. Physicochemical characterization of a dental eggshell powder abrasive material. J Appl Biomater Funct Mater 2017; 15 (04) e341-e346
    PubMedGoogle Scholar
  • 17 International Organization for Standardization. Dentistry–Dentifrices–Requirements, Test Methods and Marking. Geneva, Switzerland: ISO; 2017
    Google Scholar
  • 18 González-Cabezas C. The chemistry of caries: remineralization and demineralization events with direct clinical relevance. Dent Clin North Am 2010; 54 (03) 469-478
    CrossrefPubMedGoogle Scholar
  • 19 Pan H, Tao J, Yu X. et al. Anisotropic demineralization and oriented assembly of hydroxyapatite crystals in enamel: smart structures of biominerals. J Phys Chem B 2008; 112 (24) 7162-7165
    CrossrefPubMedGoogle Scholar
  • 20 Gronwald H, Mitura K, Volesky L. et al. The influence of suspension containing nanodiamonds on the morphology of the tooth tissue surface in atomic force microscope observations. BioMed Res Int 2018; 2018: 9856851
    CrossrefPubMedGoogle Scholar
  • 21 Nayyer M, Zahid S, Hassan SH. et al. Comparative abrasive wear resistance and surface analysis of dental resin-based materials. Eur J Dent 2018; 12 (01) 57-66
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Bozec L, Horton MA. Skeletal tissues as nanomaterials. J Mater Sci Mater Med 2006; 17 (11) 1043-1048
    CrossrefPubMedGoogle Scholar
  • 23 Gaikwad RM, Sokolov I. Silica nanoparticles to polish tooth surfaces for caries prevention. J Dent Res 2008; 87 (10) 980-983
    CrossrefPubMedGoogle Scholar
  • 24 Onwubu SC, Mdluli PS, Singh S, Lawrence M, Ngombane Y. Characterization and in vitro evaluation of an acid resistant nanosized dental eggshell-titanium dioxide material. Adv Powder Technol 2019; 30 (04) 766-773
    CrossrefGoogle Scholar
  • 25 Onwubu SC, Mdluli PS, Singh S, Nyembe S, Thakur R. An in situ evaluation of the protective effect of nano eggshell/titanium dioxide against erosive acids. International Journal of Dentistry 2018; 2018: 4216415
    PubMed
  • 26 Onwubu SC, Mdluli PS, Singh S, Tlapana T. A novel application of nano eggshell/titanium dioxide composite on occluding dentine tubules: an in vitro study. Braz Oral Res 2019; 33: e016
    CrossrefPubMedGoogle Scholar
  • 27 Onwubu SC, Mdluli PS, Singh S, Bharuth V. Remineralization potential of a modified eggshell-titanium composite-scanning electron microscope study. Eur J Dent 2019; 13 (02) 187-192
    Article in Thieme ConnectPubMedGoogle Scholar
  • 28 Onwubu SC, Mdluli PS, Singh S, Bharuth V, Makgobole MU. Evaluation of the occluding characteristics of nanosized eggshell/titanium dioxide with or without saliva. Eur J Dent 2019; 13 (04) 547-555
    Article in Thieme ConnectPubMedGoogle Scholar
  • 29 Rath SK, Sharma V, Pratap CB, Chaturvedi TP. Abrasivity of dentrifices: an update. Journal of Research in Dental Sciences. 2016; 7 (02) 96-100
    CrossrefGoogle Scholar
  • 30 Ganss C, Marten J, Hara AT, Schlueter N. Toothpastes and enamel erosion/abrasion - Impact of active ingredients and the particulate fraction. J Dent 2016; 54 (62-67) 62-67
    CrossrefPubMedGoogle Scholar
  • 31 Harris RM. A primer on colorful additives. In: Harris RM, ed. Coloring Technology for Plastics. Norwich: Williams and Andrew 1999
  • 32 Hunter ML, Addy M, Pickles MJ, Joiner A. The role of toothpastes and toothbrushes in the aetiology of tooth wear. Int Dent J 2002; 52: 399-405
    CrossrefGoogle Scholar
  • 33 Camargo IM, Saiki M, Vasconcellos MB, Avila DM. Abrasiveness evaluation of silica and calcium carbonate used in the production of dentifrices. J Cosmet Sci 2001; 52 (03) 163-167
    Google Scholar
  • 34 Moore C, Addy M. Wear of dentine in vitro by toothpaste abrasives and detergents alone and combined. J Clin Periodontol 2005; 32 (12) 1242-1246
    CrossrefPubMedGoogle Scholar
  • 35 Schemehorn BR, Moore MH, Putt MS. Abrasion, polishing, and stain removal characteristics of various commercial dentifrices in vitro. J Clin Dent 2011; 22 (01) 11-18
    PubMedGoogle Scholar
  • 36 Ferreira MC, Ramos-Jorge ML, Delbem ACB, Vieirac RdeS. Effect of Toothpastes with Different Abrasives on Eroded Human Enamel: an in situ/ex vivo study. Open Dent J 2013; 7: 132-139
    CrossrefPubMedGoogle Scholar
  • 37 Athawale R, Srinath SK, Chowdary C. Comparative evaluation of enamel abrasivity of different commercially available dentifrices-An in vitro study. Journal of Indian Association of Public Health Dentistry. 2018; 16 (01) 78-82
    CrossrefGoogle Scholar
  • 38 Wiegand A, Schlueter N. The role of oral hygiene: does toothbrushing harm?. Monogr Oral Sci 2014; 25: 215-219
    CrossrefPubMedGoogle Scholar