CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(02): 253-258
DOI: 10.1055/s-0040-1717053
Original Article

Evaluation of 2% Chlorhexidine and 2% Sodium Fluoride as Endodontic Irrigating Solutions on Root Dentine Microhardness: An In Vitro Study

Sangeeta Kulkarni
1   Department of Conservative Dentistry and Endodontics, Al-Badar Dental College and Hospital, Kalaburagi, Karnataka, India
,
2   Department of Conservative Dental Sciences, College of Dentistry, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia
,
Kiran Ghatole
1   Department of Conservative Dentistry and Endodontics, Al-Badar Dental College and Hospital, Kalaburagi, Karnataka, India
,
Ali Robaian AlQahtani
2   Department of Conservative Dental Sciences, College of Dentistry, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia
,
Faris Yahya I. Asiri
3   Department of Preventive Dental Sciences, College of Dentistry, King Faisal University, Al-Ahsa, Saudi Arabia
,
Ziyad Ibrahim Alghomlas
4   General Dental Practitioner, Riyadh, Saudi Arabia
,
Tameem Abdullah Alothman
5   General Dental Practitioner, Al-Delam, Saudi Arabia
,
Fawaz Fahad Alhajri
6   General Dental Practitioner, Al-Kharj, Saudi Arabia
› Author Affiliations

Abstract

Objectives The aim of this study was to evaluate the effects of 2% chlorhexidine gluconate (CHG) and 2% sodium fluoride (NaF) as endodontic irrigants on microhardness of root dentin.

Materials and Methods In this in vitro study, access cavity and root canal preparations were done on 24 freshly extracted anterior teeth. After sectioning into 24 dentin discs using hard tissue microtome in 2 mm thickness, all samples were immersed in solutions of 17% ethylenediamine tetra-acetic acid (EDTA) (2 minutes) followed by 2.5% of sodium hypochlorite (NaOCl) (10 minutes). Then samples were randomly divided into three groups based on the irrigant used: Group I: saline (control group); Group II: 2% NaF; Group III: 2% CHG for two minutes each. Dentin microhardness was measured before (pretreatment), during (after treatment with 17% EDTA and 2.5% NaOCl), and after the experimental period (after treatment with saline, 2% NaF, and 2% CHG) using a Vickers indenter. Statistical evaluation of the data was done using one-way analysis of variance (ANOVA) and the Student’s t-test, and the values are tabulated.

Results Specimens rinsed in 2% CHG showed a significant increase in Vickers hardness number (VHN) values (p < 0.05), as compared with EDTA and NaOCl groups, whereas saline and 2% NaF groups showed no significant difference.

Conclusions NaF did not show any significant effect on microhardness of the root dentin. CHG as an irrigant was seen to have a strengthening effect on dentin microhardness in comparison to NaOCl and EDTA, which has decreased the strength of root dentin.



Publication History

Article published online:
08 October 2020

© 2020. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • References

  • 1 Kishen A. Mechanisms and risk factors for fracture predilection in endodontically treated teeth. Endod Topics 2006; 13: 57-83
  • 2 Saleh AA, Ettman WM. Effect of endodontic irrigation solutions on microhardness of root canal dentine. J Dent 1999; 27 (01) 43-46
  • 3 Shabbir J, Qazi F, Farooqui W, Ahmed S, Zehra T, Khurshid Z. Effect of Chinese propolis as an intracanal medicament on post-operative endodontic pain: a double-blind randomized controlled trial. Int J Environ Res Public Health 2020; 17 (02) 445
  • 4 Siqueira Jr JF, Rôças IN. Clinical implications and microbiology of bacterial persistence after treatment procedures. J Endod 2008; 34 (11) 1291-1301.e3
  • 5 Wong R. Conventional endodontic failure and retreatment. Dent Clin North Am 2004; 48 (01) 265-289
  • 6 Basmadjian-Charles CL, Farge P, Bourgeois DM, Lebrun T. Factors influencing the long-term results of endodontic treatment: a review of the literature. Int Dent J 2002; 52 (02) 81-86
  • 7 Gutarts R, Nusstein J, Reader A, Beck M. In vivo debridement efficacy of ultrasonic irrigation following hand-rotary instrumentation in human mandibular molars. J Endod 2005; 31 (03) 166-170
  • 8 Svec TA, Harrison JW. Chemomechanical removal of pulpal and dentinal debris with sodium hypochlorite and hydrogen peroxide vs normal saline solution. J Endod 1977; 3 (02) 49-53
  • 9 Walton RE. Histologic evaluation of different methods of enlarging the pulp canal space. J Endod 1976; 2 (10) 304-311
  • 10 Haapasalo M, Shen Y, Wang Z, Gao Y. Irrigation in endodontics. Br Dent J 2014; 216 (06) 299-303
  • 11 Ali MA, Kano B. Endodontic materials: from old materials to recent advances. Advanced Dental Biomaterials 2019; 255-299
  • 12 Mustafa M, Alaajam WH, Azeim AA, Alfayi NA, Alqobty RM, Alghannam S. Diffusion of calcium hydroxide through dentinal tubules of retreated root canals: an in vitro study. Eur J Dent 2018; 12 (03) 386-392
  • 13 Podar R, Kulkarni GP, Dadu SS, Singh S, Singh SH. In vivo antimicrobial efficacy of 6% Morinda citrifolia, Azadirachta indica, and 3% sodium hypochlorite as root canal irrigants. Eur J Dent 2015; 9 (04) 529-534
  • 14 Goldsmith M, Gulabivala K, Knowles JC. The effect of sodium hypochlorite irrigant concentration on tooth surface strain. J Endod 2002; 28 (08) 575-579
  • 15 Mohammadi Z, Shalavi S, Jafarzadeh H. Ethylenediaminetetraacetic acid in endodontics. Eur J Dent 2013; 7 (Suppl. 01) S135-S142
  • 16 Grande NM, Plotino G, Falanga A, Pomponi M, Somma F. Interaction between EDTA and sodium hypochlorite: a nuclear magnetic resonance analysis. J Endod 2006; 32 (05) 460-464
  • 17 Yassen GH, Al-Angari SS, Platt JA. The use of traditional and novel techniques to determine the hardness and indentation properties of immature radicular dentin treated with antibiotic medicaments followed by ethylenediaminetetraacetic acid. Eur J Dent 2014; 8 (04) 521-527
  • 18 Leonardo MR, Tanomaru Filho M, Silva LA, Nelson Filho P, Bonifácio KC, Ito IY. In vivo antimicrobial activity of 2% chlorhexidine used as a root canal irrigating solution. J Endod 1999; 25 (03) 167-171
  • 19 Siqueira Jr JF, Paiva SS, Rôças IN. Reduction in the cultivable bacterial populations in infected root canals by a chlorhexidine-based antimicrobial protocol. J Endod 2007; 33 (05) 541-547
  • 20 Vieira A, Hancock R, Dumitriu M, Schwartz M, Limeback H, Grynpas M. How does fluoride affect dentin microhardness and mineralization?. J Dent Res 2005; 84 (10) 951-957
  • 21 Sivapriya E, Sridevi K, Periasamy R, Lakshminarayanan L, Pradeepkumar AR. Remineralization ability of sodium fluoride on the microhardness of enamel, dentin, and dentinoenamel junction: an in vitro study. J Conserv Dent 2017; 20 (02) 100-104
  • 22 Martin JMH, de Almeida JB, Rosa EAR. et al. Effect of fluoride therapies on the surface roughness of human enamel exposed to bleaching agents. Quintessence Int 2010; 41 (01) 71-78
  • 23 Gomes-Filho JE, Aurélio KG, Costa MM, Bernabé PF. Comparison of the biocompatibility of different root canal irrigants. J Appl Oral Sci 2008; 16 (02) 137-144
  • 24 Ari H, Erdemir A, Belli S. Evaluation of the effect of endodontic irrigation solutions on the microhardness and the roughness of root canal dentin. J Endod 2004; 30 (11) 792-795
  • 25 Dioguardi M, Gioia GD, Illuzzi G, Laneve E, Cocco A, Troiano G. Endodontic irrigants: different methods to improve efficacy and related problems. Eur J Dent 2018; 12 (03) 459-466
  • 26 Pascon FM, Kantovitz KR, Sacramento PA, Nobre-dos-Santos M, Puppin-Rontani RM. Effect of sodium hypochlorite on dentine mechanical properties. A review. J Dent 2009; 37 (12) 903-908
  • 27 Grigoratos D, Knowles J, Ng YL, Gulabivala K. Effect of exposing dentine to sodium hypochlorite and calcium hydroxide on its flexural strength and elastic modulus. Int Endod J 2001; 34 (02) 113-119
  • 28 Pinheiro SL, Silva CC, Silva LAD. et al. Antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water as irrigants in mesiobuccal root canals with severe curvature of mandibular molars. Eur J Dent 2018; 12 (01) 94-99
  • 29 Moon PC, Weaver J, Brooks CN. Review of matrix metalloproteinases’ effect on the hybrid dentin bond layer stability and chlorhexidine clinical use to prevent bond failure. Open Dent J 2010; 4: 147-152
  • 30 Mohammadi Z, Abbott PV. The properties and applications of chlorhexidine in endodontics. Int Endod J 2009; 42 (04) 288-302