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CC BY-NC-ND 4.0 · Arquivos Brasileiros de Neurocirurgia: Brazilian Neurosurgery 2023; 42(01): e14-e18
DOI: 10.1055/s-0041-1731065
Original Article

Aquaporin-4 Expression in Meningioma Malignancy Progression

Expressão da aquaporina-4 na progressão da malignidade dos meningiomas*
Benedito Jamilson Araújo Pereira 
1   Department of Neurology, Hospital das Clínicas, Universidade de São Paulo, São Paulo, SP, Brazil
,
Sueli M. Oba-Shinjo 
2   Department of Neurology, Laboratório de Biologia Celular e Molecular LIM15, Escola de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
,
Paulo Henrique Pires de Aguiar 
3   Centro Universitário Faculdade de Medicina do ABC, Santo André, Brazil
4   Pontifícia Universidade Católica de São Paulo, Sorocaba, Brazil
,
Antonio Nogueira de Almeida 
1   Department of Neurology, Hospital das Clínicas, Universidade de São Paulo, São Paulo, SP, Brazil
5   IPQ Functional Neurosurgery Division, Hospital das Clínicas, Universidade de São Paulo, São Paulo, SP, Brazil
,
Wellingson da Silva Paiva 
1   Department of Neurology, Hospital das Clínicas, Universidade de São Paulo, São Paulo, SP, Brazil
,
Suely Kazue Nagahashi Marie 
1   Department of Neurology, Hospital das Clínicas, Universidade de São Paulo, São Paulo, SP, Brazil
2   Department of Neurology, Laboratório de Biologia Celular e Molecular LIM15, Escola de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil
6   Center for the Study of Cellular and Molecular Therapy, Universidade de São Paulo, São Paulo, SP, Brazil
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Abstract

Objectives The aim of the present study is to analyze if aquaporin-4 (AQP4) may also be a tumor progression marker for meningiomas.

Methods This is an immunohistochemistry study realized at the Universidade de São Paulo, São Paulo, state of São Paulo, Brazil: frozen meningioma samples from 81 patients (57 females and 24 males, age range from 22 to 81 years old, average 56.5 ± 14.1 years old), including 57 meningiomas World Health Organization (WHO) grade I (GI); 19 grade II (GII), and 5 grade III (GIII) were analyzed. The relative expression level of AQP4 was analyzed by quantitative polymerase chain reaction (qPCR), using the SYBR Green approach and for staining detection. Tissue sections were routinely processed and subjected to antigen retrieval.

Results The expression of AQP4 in meningioma samples ranged from 0 to 10.26, with a median of 0.001 in GI cases, of 0.008 in GII cases, and of 0.006 in GIII cases. Although not statistically significant (p = 0.942), GI meningiomas have a lower median AQP4 expression level than higher malignant grade cases.

Conclusion The AQP4 gene and protein expressions presented no association with meningioma malignant progression.

Resumo

Objetivo O objetivo do presente estudo é analisar se a aquaporina-4 (AQP4) também pode ser um marcador de progressão tumoral para meningiomas.

Métodos Trata-se de um estudo imunohistoquímico realizado na Universidade de São Paulo, SP, Brasil. Amostras congeladas de meningioma de 81 pacientes (57 mulheres e 24 homens, faixa etária de 22 a 81 anos, média de 56,5 ± 14,1 anos), incluindo 57 meningiomas grau I (GI) da Organização Mundial da Saúde (OMS); 19 grau II (GII) e 5 grau III (GIII) foram analisados. O nível de expressão relativa de AQP4 foi analisado por reação em cadeia de polimerase quantitativa (qPCR, sigla em inglês), usando a abordagem SYBR Green e para detecção de manchas. As seções de tecido foram rotineiramente processadas e sujeitas a recuperação de antígeno.

Resultados A expressão de AQP4 em amostras de meningioma variou de 0 a 10,26, com mediana de 0,001 nos casos GI; 0,008 nos casos GII; e 0,006 nos casos GIII. Embora não sejam estatisticamente significantes (p = 0,942), os meningiomas GI apresentam mediana mais baixa do nível de expressão de AQP4 do que os casos de grau maligno mais alto.

Conclusão Expressões de genes e proteínas AQP4 apresentadas na associação com progressão maligna do meningioma.

Keywords

aquaporin-4 - malignancy - meningioma

Palavras-chave

aquaporina-4 - malignidade - meningioma

Disclosure

The authors have no personal financial or institutional interest in any of the drugs, materials, or devices described in this article.


Ethical Approval

All procedures performed in the present study were in accordance with the ethical standards of the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The present study was approved by our Institutional Review Board under the registration number CAPPESq # 200/05.


Informed Consent

All authors agree to the publication guidelines of the São Paulo medical Journal


The present article does not contain any studies with human participants performed by any of the authors.


* The present study was performed at the LIM 15 – Hospital de Clínicas da Universidade de São Paulo (USP).




Publication History

Received: 08 June 2020

Accepted: 09 March 2021

Article published online:
07 November 2022

© 2022. Sociedade Brasileira de Neurocirurgia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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