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CC BY 4.0 · Eur J Dent 2022; 16(01): 122-129
DOI: 10.1055/s-0041-1731886
Original Article

Preliminary Histological Evaluation of the Application of Ozone in the First Days of Orthodontic Force Induction in Animal Model

Melissa Faccini
1   Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil
,
Felipe Agostini
1   Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil
,
Tassio Drieu
2   Department of Dentistry, São Leopoldo Mandic, Campinas, Brazil
,
Francisco Ubiratan Ferreira de Campos
3   Department of Oral Microbiology, São Leopoldo Mandic, Campinas, Brazil
,
Aguinaldo Garcez
3   Department of Oral Microbiology, São Leopoldo Mandic, Campinas, Brazil
,
Glauber Fabre Carinhena
4   Department of Orthodontics, Superior Dentistry School, Brazil
,
Samira Salmeron
5   Department of Periodontics, Ingá University Center UNINGA, Maringá, Brazil
,
Ana Regina Casaroto
6   Department of Pathology, Ingá University Center UNINGA, Maringá, Brazil
,
Fabricio Pinelli Valarelli
7   Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil
,
Karina Maria Salvatore Freitas 
7   Department of Orthodontics, Ingá University Center UNINGA, Maringá, Brazil
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Abstract

Objectives The aim of the study was to histologically evaluate the effect of ozone therapy on orthodontic force induction in an animal model.

Materials and Methods Twenty-four Wistar rats were divided into three groups (n = 8). A NiTi coil spring was installed from the maxillary first molar to the maxillary central incisor. G1 was control and G2/G3 received 1 mL of ozonated gas at concentrations of 10 and 60 µg/mL, in the buccal mucosa above the first molar roots. The animals were euthanized 3 and 5 days after the procedure. Histological sections were obtained, longitudinally of the first molar’ long axis, in the mesiodistal direction. The number of osteoclasts, osteoblasts, blood vessels, polymorphonuclear and mononuclear cells, formation of osteoid tissue and hyaline areas, and root resorption were evaluated with light microscope, in tension and pressure sides. Intergroup comparisons were performed with Kruskal–Wallis, Dunn, and Chi-square tests.

Results At 3-days pressure side, a greater number of osteoclasts was observed in ozone groups and greater number of blood vessels and polymorphonuclear cells were observed in G2. On the tension side, there was a significantly greater number of blood vessels, osteoblasts, and mononuclear cells in G2. At 5-days pressure side, there was a significantly greater number of osteoclasts in G2, blood vessels and osteoblasts in the ozone groups, and lesser number of polymorphonuclear cells in G3.

Conclusion Ozone therapy increased the number of osteoclasts on the pressure side and osteoblasts on tension side, in 10 µg/mL concentration, demonstrating histological parameters favorable to bone remodeling. The 60 µg/mL ozone concentration accelerated the periodontal ligament reorganization process.

Keywords

orthodontic movement - ozone - orthodontics - ozone therapy


Publication History

Article published online:
24 August 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).

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