Download PDF
CC BY 4.0 · Glob Med Genet 2022; 09(02): 118-123
DOI: 10.1055/s-0041-1740019
Original Article

Molecular Detection of blaOXA-type Carbapenemase Genes and Antimicrobial Resistance Patterns among Clinical Isolates of Acinetobacter baumannii

Maghsoud Kafshnouchi
1   Department of Microbiology, Maragheh Islamic Azad University, Maragheh, Iran
,
Marzieh Safari
2   Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
,
Amir Khodavirdipour 
3   Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
,
Abbas Bahador
4   Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran
,
Seyed Hamid Hashemi
5   Department of Infectious Diseases, Hamadan University of Medical Sciences, Hamadan, Iran
,
Mohammad Sina Alikhani
6   Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
,
Massoud Saidijam
7   Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
,
Mohammad Yousef Alikhani 
2   Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
› Author Affiliations Funding The present study was supported financially by the Vice-Chancellor of Research and Technology, Hamadan University of Medical Sciences, Hamadan, Iran.
› Further Information
   Permissions and Reprints

Abstract

Acinetobacter baumannii is a bacterium found in most places, especially in clinics and hospitals, and an important agent of nosocomial infections. The presence of class D enzymes such as OXA-type carbapenemases in A. baumannii is proven to have a key function in resistance to carbapenem. The aim of the current study is to determine the blaOXA-type carbapenemase genes and antimicrobial resistance among clinically isolated samples of A. baumannii. We assessed 100 clinically isolated specimens of A. baumannii from patients in intensive care units of educational hospitals of Hamadan, West of Iran. The A. baumannii isolates' susceptibility to antibiotics was performed employing disk diffusion method. Multiplex polymerase chain reaction was used to identify the blaOXA-24-like , blaOXA-23-like , blaOXA-58-like , and blaOXA-51-like genes. The blaOXA-23-like , blaOXA-24-like , and blaOXA-58-like genes' prevalence were found to be 84, 58, and 3%, respectively. The highest coexistence of the genes was for blaOXA-51/23 (84%) followed by blaOXA-51/24-like (58%). The blaOXA-51/23- like pattern of genes is a sort of dominant gene in resistance in A. baumannii from Hamadan hospitals. The highest resistance to piperacillin (83%) and ciprofloxacin (81%) has been observed in positive isolates of blaOXA-23-like . The A. baumannii isolates with blaOXA-58-like genes did not show much resistance to antibiotics. Based on the results of the phylogenetic tree analysis, all isolates have shown a high degree of similarity. This study showed the high frequency of OXA-type carbapenemase genes among A. baumannii isolates from Hamadan hospitals, Iran. Thus, applying an appropriate strategy to limit the spreading of these strains and also performing new treatment regimens are necessary.

Keywords

Acinetobacter baumannii - nosocomial infection - OXA-type carbapenemase

Consent for Publication

All authors consented for publication of the above paper.


Availability of Data and Material

All data generated or analyzed during this study are included in this published article.


Authors' Contributions

M.Y.A. designed the study, edited the manuscript. M.S. and A.B. were scientific advisor on molecular genetics section. M.K., M.S., and M.S.A. collaborated in collecting samples and doing experiments. S.H.H. collaborated on patient introduction and clinical examination. A.K. wrote the scientific draft and checked the English use and grammar of the manuscript.




Publication History

Received: 20 September 2021

Accepted: 18 October 2021

Article published online:
02 December 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA. Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 2007; 51 (10) 3471-3484
    CrossrefPubMedGoogle Scholar
  • 2 Yeom J, Shin JH, Yang JY, Kim J, Hwang GS. (1)H NMR-based metabolite profiling of planktonic and biofilm cells in Acinetobacter baumannii 1656-2. PLoS One 2013; 8 (03) e57730
    CrossrefPubMedGoogle Scholar
  • 3 Poirel L, Bonnin RA, Nordmann P. Genetic basis of antibiotic resistance in pathogenic Acinetobacter species. IUBMB Life 2011; 63 (12) 1061-1067
    CrossrefPubMedGoogle Scholar
  • 4 Gulati S, Kapil A, Das B, Dwivedi SN, Mahapatra AK. Nosocomial infections due to Acinetobacter baumannii in a neurosurgery ICU. Neurol India 2001; 49 (02) 134-137
    PubMedGoogle Scholar
  • 5 Rodríguez Guardado A, Maradona JA, Asensi V. et al. [Postsurgical meningitis caused by Acinetobacter baumannii: study of 22 cases and review of the literature]. Rev Clin Esp 2001; 201 (09) 497-500
    CrossrefPubMedGoogle Scholar
  • 6 Wang KW, Chang WN, Huang CR. et al. Post-neurosurgical nosocomial bacterial meningitis in adults: microbiology, clinical features, and outcomes. J Clin Neurosci 2005; 12 (06) 647-650
    CrossrefPubMedGoogle Scholar
  • 7 Krol V, Hamid NS, Cunha BA. Neurosurgically related nosocomial Acinetobacter baumannii meningitis: report of two cases and literature review. J Hosp Infect 2009; 71 (02) 176-180
    CrossrefPubMedGoogle Scholar
  • 8 Poirel L, Naas T, Nordmann P. Diversity, epidemiology, and genetics of class D beta-lactamases. Antimicrob Agents Chemother 2010; 54 (01) 24-38
    CrossrefPubMedGoogle Scholar
  • 9 Shahcheraghi F, Aslani MM, Mahmoudi H. et al. Molecular study of carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenems and determining their clonal relationship using pulsed-field gel electrophoresis. J Med Microbiol 2017; 66 (05) 570-576
    CrossrefPubMedGoogle Scholar
  • 10 Bertini A, Poirel L, Mugnier PD, Villa L, Nordmann P, Carattoli A. Characterization and PCR-based replicon typing of resistance plasmids in Acinetobacter baumannii. Antimicrob Agents Chemother 2010; 54 (10) 4168-4177
    CrossrefPubMedGoogle Scholar
  • 11 Opazo A, Domínguez M, Bello H, Amyes SG, González-Rocha G. OXA-type carbapenemases in Acinetobacter baumannii in South America. J Infect Dev Ctries 2012; 6 (04) 311-316
    CrossrefPubMedGoogle Scholar
  • 12 Brown S, Young HK, Amyes SG. Characterisation of OXA-51, a novel class D carbapenemase found in genetically unrelated clinical strains of Acinetobacter baumannii from Argentina. Clin Microbiol Infect 2005; 11 (01) 15-23
    CrossrefPubMedGoogle Scholar
  • 13 Higgins PG, Poirel L, Lehmann M, Nordmann P, Seifert H. OXA-143, a novel carbapenem-hydrolyzing class D beta-lactamase in Acinetobacter baumannii. Antimicrob Agents Chemother 2009; 53 (12) 5035-5038
    CrossrefPubMedGoogle Scholar
  • 14 Howard A, O'Donoghue M, Feeney A, Sleator RD. Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence 2012; 3 (03) 243-250
    CrossrefPubMedGoogle Scholar
  • 15 Safari M, Mozaffari Nejad AS, Bahador A, Jafari R, Alikhani MY. Prevalence of ESBL and MBL encoding genes in Acinetobacter baumannii strains isolated from patients of intensive care units (ICU). Saudi J Biol Sci 2015; 22 (04) 424-429
    CrossrefPubMedGoogle Scholar
  • 16 Turton JF, Woodford N, Glover J, Yarde S, Kaufmann ME, Pitt TL. Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species. J Clin Microbiol 2006; 44 (08) 2974-2976
    CrossrefPubMedGoogle Scholar
  • 17 CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 28th edition.. CLSI Supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2018
    Google Scholar
  • 18 Woodford N, Ellington MJ, Coelho JM. et al. Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents 2006; 27 (04) 351-353
    CrossrefPubMedGoogle Scholar
  • 19 Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10 (03) 512-526
    PubMedGoogle Scholar
  • 20 Queenan AM, Bush K. Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 2007; 20 (03) 440-458 table of contents
    CrossrefPubMedGoogle Scholar
  • 21 Walther-Rasmussen J, Høiby N. OXA-type carbapenemases. J Antimicrob Chemother 2006; 57 (03) 373-383
    CrossrefPubMedGoogle Scholar
  • 22 Feizabadi MM, Fathollahzadeh B, Taherikalani M. et al. Antimicrobial susceptibility patterns and distribution of blaOXA genes among Acinetobacter spp. isolated from patients at Tehran hospitals. Jpn J Infect Dis 2008; 61 (04) 274-278
    CrossrefPubMedGoogle Scholar
  • 23 Zafari M, Feizabadi MM, Jafari S, Abdollahi A, Sabokbar A. High prevalence of OXA-type carbapenemases among Acinetobacter baumannii strains in a teaching hospital of Tehran. Acta Microbiol Immunol Hung 2017; 64 (04) 385-394
    CrossrefPubMedGoogle Scholar
  • 24 Safari M, Saidijam M, Bahador A, Jafari R, Alikhani MY. High prevalence of multidrug resistance and metallo-beta-lactamase (MβL) producing Acinetobacter baumannii isolated from patients in ICU wards, Hamadan, Iran. J Res Health Sci 2013; 13 (02) 162-167
    PubMedGoogle Scholar
  • 25 Bardbari AM, Arabestani MR, Karami M. et al. Highly synergistic activity of melittin with imipenem and colistin in biofilm inhibition against multidrug-resistant strong biofilm producer strains of Acinetobacter baumannii. Eur J Clin Microbiol Infect Dis 2018; 37 (03) 443-454
    CrossrefPubMedGoogle Scholar
  • 26 Zowawi HM, Sartor AL, Sidjabat HE, Balkhy HH, Walsh TR, Al Johani SM. et al. Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii isolates in the Gulf Cooperation Council States: dominance of OXA-23-type producers. J Clin Microbiol 2015; 53: 896-903
    CrossrefPubMedGoogle Scholar
  • 27 Merino M, Poza M, Roca I. et al. Nosocomial outbreak of a multiresistant Acinetobacter baumannii expressing OXA-23 carbapenemase in Spain. Microb Drug Resist 2014; 20 (04) 259-263
    CrossrefPubMedGoogle Scholar
  • 28 Dettori M, Piana A, Deriu MG. et al. Outbreak of multidrug-resistant Acinetobacter baumannii in an intensive care unit. New Microbiol 2014; 37 (02) 185-191
    PubMedGoogle Scholar
  • 29 Kooti S, Motamedifar M, Sarvari J. Antibiotic resistance profile and distribution of oxacillinase genes among clinical isolates of Acinetobacter baumannii in Shiraz teaching hospitals, 2012–2013. Jundishapur J Microbiol 2015; 8 (08) e20215
    CrossrefPubMedGoogle Scholar