Hypoxia and Hyperoxia Affect Serum Angiogenic Regulators in T2DM Men during Cycling

Christian Brinkmann; Alexander Metten; Philipp Scriba; Christos V. M. Tagarakis; Patrick Wahl; Jo Latsch; Klara Brixius; Wilhelm Bloch

doi:10.1055/s-0042-116823

International Journal of Sports Medicine, Inhaltsverzeichnis

Int J Sports Med 2017; 38(02): 92-98
DOI: 10.1055/s-0042-116823

Physiology & Biochemistry

Hypoxia and Hyperoxia Affect Serum Angiogenic Regulators in T2DM Men during Cycling

Christian Brinkmann

¹Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

,

Alexander Metten

¹Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

,

Philipp Scriba

¹Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

,

Christos V. M. Tagarakis

¹Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

,

Patrick Wahl

²Institute of Training Science and Sport Informatics, German Sport University Cologne, Cologne, Germany

,

Jo Latsch

³Department of Preventive and Rehabilitative Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

,

Klara Brixius

¹Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

,

Wilhelm Bloch

¹Department of Molecular and Cellular Sport Medicine, Institute of Cardiovascular Research and Sport Medicine, German Sport University Cologne, Cologne, Germany

› Institutsangaben

Abstract

Exercise-induced transient increases in pro-angiogenic regulators can promote angiogenesis.This pilot study aims to analyze the potential of exercise to positively affect angiogenic regulators in patients with type 2 diabetes mellitus (T2DM), who often exhibit abnormal angiogenesis, under different environmental conditions. 9 overweight/obese men with uncomplicated T2DM (8 took anti-diabetic drugs) performed submaximal cycling for 40 min in normoxia (≈21 vol%O₂), hypoxia (≈14 vol%O₂) and during alternating hypoxia/hyperoxia (≈14 vol%O₂/≈30 vol%O₂, 5-min intervals) (3×3 crossover design). Serum pro-angiogenic vascular endothelial growth factor (VEGF), matrix metalloproteinase (MMP)-2, MMP-9 and anti-angiogenic endostatin were quantified using enzyme-linked immunosorbent assay (ELISA) kits. Non-parametric statistical tests (Wilcoxon, Friedman analysis of variance) were applied. VEGF increased significantly from pre- to post-exercise with hypoxia and hypoxia/hyperoxia. MMP-2 increased significantly in all experimental runs, while MMP-9 only increased significantly with hypoxia and hypoxia/hyperoxia. Endostatin increased significantly with normoxia and hypoxia. However, the magnitude of changes did not differ significantly between conditions. Capillary blood lactate was significantly lower following cycling with hypoxia/hyperoxia than with hypoxia alone. Although differences in subjective ratings of perceived exertion failed significance, 7 subjects were less exerted with hypoxia/hyperoxia than with hypoxia. Submaximal cycling with hypoxia or alternating hypoxia/hyperoxia may induce a more reliable up-regulation of pro-angiogenic regulators compared with normoxia, while hypoxia/hyperoxia may be better tolerated than hypoxia alone.

Key words

type 2 diabetes - exercise - physical activity - cycling

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