Drug Res (Stuttg) 2017; 67(09): 509-514
DOI: 10.1055/s-0042-119647
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Chrysin Alters microRNAs Expression Levels in Gastric Cancer Cells: Possible Molecular Mechanism

Farideh Mohammadian1, 2, Younes Pilehvar-Soltanahmadi1, 2, Shahriar Alipour3, Mehdi Dadashpour1, 2, Nosratollah Zarghami1, 2
  • 1Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • 2Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
  • 3Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Further Information

Publication History

received 12 August 2016

accepted 19 October 2016

Publication Date:
19 June 2017 (eFirst)

Abstract

Background Gastric carcinoma still remains the second most common cause of cancer mortality in the world. Chrysin, as a flavone, has showed cancer chemopreventive activity. The cellular and molecular mechanisms of chrysin in cancer cells have not been fully understood.

Objective In this study, we investigate expression levels of let-7a, miR-9, mir-18a, miR-21, miR-22, miR-34a, miR-126 and mir-221 to describe the anti-cancer effects of chrysin.

Materials and Methods The cytotoxic effects of chrysin were assessed using MTT assay. The effect of chrysin on the microRNAs expression was determined by qRT-PCR.

Results The MTT results for different concentrations of chrysin at different times on the Gastric carcinoma cells showed that IC50 for chrysin was 68.24 µM after 24 h of treatment. Expression analysis identified that miR-18, miR-21 and miR-221 were down regulated whereas let-7a, miR-9, miR-22, miR-34a and miR-126 were up regulated in Gastric carcinoma cell line (p<0.05).

Conclusion Treatment with chrysin can alter the miRNAs expression and these findings might be an explanation for molecular mechanism of chrysin effect on gastric cancer.