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CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2022; 12(04): 454-458
DOI: 10.1055/s-0042-1742367
Brief Report

A Preliminary Assessment of Tinospora sinensis on Mice Liver

Abidah Parveen 
1   Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, United States
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, United States
3   Department of Pharmaceutical Sciences, Abbottabad University of Science and Technology, Havelian, Khyber Pakhtunkhwa, Pakistan
,
Tahir Maqbool Mir 
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, United States
,
Zulfiqar Ali
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, United States
,
Ikhlas A. Khan
1   Division of Pharmacognosy, Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, University, MS, United States
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, United States
,
M. Khalid Ashfaq
2   National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS, United States
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Abstract

Objective A preliminary study was conducted to assess the role of Tinospora sinensis extract on liver in mice in normal and lipopolysaccharide (LPS)-induced health-compromised conditions.

Method Mice (n = 3–5) were randomly assigned into groups I to IV for hepatotoxic studies. Group I was assigned normal, group II was given LPS (6 mg/kg, intraperitoneal [ip]), group III was given T. sinensis only (1 g/kg/day for 21 days), whereas group IV was administered T. sinensis (1 g/kg/day per os [po] for 21 days) with LPS (6 mg/kg ip given on 7th day). Group V received monocrotaline (MCT) (200 mg/kg, p.o.) only. Group VI received MCT (200 mg/kg, po) and LPS (6 mg/kg ip). Group VII was given T. sinensis (500 mg/kg/day po for 7 days) followed by MCT (200 mg/kg, p.o.) and LPS (6 mg/kg, ip) on the 7th day. Groups V to VII were used to assess the effect of T. sinensis in MCT + LPS-induced hepatotoxicity model.

Results No elevation in alanine transaminase (ALT) levels was observed in mice treated with T. sinensis in group III or group IV compared with normal (vehicle treated) group (group I). Elevation in ALT levels was observed in group VI (MCT + LPS) and group VII; histopathology showed liver injury. Pretreatment of mice with T. sinensis (group VII) did not show any reduction in the elevated ALT levels.

Conclusions In the preliminary assessment, T. sinensis extract was found to exhibit neither hepatotoxicity itself nor the potential to thwart liver damage by a xenobiotic under the given test conditions, dosage, and duration of the study.

Keywords

Tinospora sinensis - extract - lipopolysaccharide - xenobiotic


Publication History

Article published online:
15 February 2022

© 2022. Nitte (Deemed to be University). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • References

  • 1 Cooke T. Flora of the Presidency of Bombay Vol 1. London: Taylor and Francis; 1901
    Google Scholar
  • 2 Udayan PS, George S, Tushar KV, Balachandran I. Tinospora sinensis (Lour.) Merr. from Sickupara, Kolli Hills forest, Namakkal District, Tamil Nadu. Zoos' Print J 2004; 19 (09) 1622-1623
    CrossrefGoogle Scholar
  • 3 Liu YH, Luo XR, Wu RF, Zhang BN. Flora of China (Zhongguo Zhiwu Zhi). Beijing: Science Press 1996; 30 (01) 305
    Google Scholar
  • 4 Sankar RV, Ravikumar K, Ravichandran P. Tinospora sinensis (Lour.) Merr. (Menispermaceae) - a hitherto unreported red listed medicinal plant from Tamil Nadu State. Indian For 2004; 130 (07) 731-734
    Google Scholar
  • 5 Li RW, David Lin G, Myers SP, Leach DN. Anti-inflammatory activity of Chinese medicinal vine plants. J Ethnopharmacol 2003; 85 (01) 61-67
    CrossrefPubMedGoogle Scholar
  • 6 Chopra RN, Nayer SLCI. Glossary of Indian Medicinal Plants. New Delhi, India: Council of Scientific and Industrial Research; 1956
    Google Scholar
  • 7 Wealth of India. New Delhi: Raw materials, Vol. X. Publication and Information Directorate, CSIR; 1976
    PubMedGoogle Scholar
  • 8 Sereena K, Remashree AB, Vemballur P. Histological, histochemical and phytochemical studies of the raw drug amrita from different raw drug markets of Kerala. Int J Interdiscip Multidiscip Stud 2014; 1 (05) 182-191
    Google Scholar
  • 9 Choudhary N, Siddiqui MB, Khatoon S. Pharmacognostic evaluation of Tinospora cordifolia (Willd.) Miers and identification of biomarkers. Indian J Tradit Knowl 2014; 13 (03) 543-550
    Google Scholar
  • 10 Chavan T, Khadke S, Harke S. et al. Satwa from three Tinospora species exhibits differential hepatoprotective activity against repeated acetaminophen dosing in rats. J Pharm Res 2013; 6 (01) 123-128
    Google Scholar
  • 11 Dahanukar SA, Thatte UM, Rege NN. Immunostimulants in Ayurveda medicine. In: Immunomodulatory Agents from Plants. Progress in Inflammation Research. Basel: Birkhäuser Basel; 1999
    CrossrefGoogle Scholar
  • 12 Rege NN, Thatte UM, Dahanukar SA. Adaptogenic properties of six rasayana herbs used in Ayurvedic medicine. Phytother Res 1999; 13 (04) 275-291
    CrossrefPubMedGoogle Scholar
  • 13 Agarwal A, Malini S, Bairy K, Rao MS. Effect of Tinospora cordifolia on learning and memory in normal and memory deficit rats. Indian J Pharmacol 2002; 34: 339-349
    Google Scholar
  • 14 Nagarkar B. Comparative hepatoprotective potential of Tinospora cordifolia, Tinospora sinensis and Neem-guduchi. Br J Pharm Res 2013; 3 (04) 906-916
    CrossrefGoogle Scholar
  • 15 Chavan T, Ghadge A, Karandikar M. et al. Hepatoprotective activity of satwa, an ayurvedic formulation, against alcohol-induced liver injury in rats. Altern Ther Health Med 2017; 23 (04) 34-40
    PubMedGoogle Scholar
  • 16 Narendra ND, Salma FS, Khandekar D. et al. Evaluation of hepato protective activity of ethanolic root extract of Tinospora sinensis . Int J Biol Pharm Res 2013; 4 (12) 1065-1069
    Google Scholar
  • 17 Wu M, Deng JYC. Clinical toxicology,. In: XXXIII International Congress of the European Association of Poisons Centres and Clinical Toxicologists (EAPCCT). Copenhagen, Denmark: 2013: 268
    Google Scholar
  • 18 Hamesch K, Borkham-Kamphorst E, Strnad P, Weiskirchen R. Lipopolysaccharide-induced inflammatory liver injury in mice. Lab Anim 2015; 49 (1, Suppl): 37-46
    CrossrefPubMedGoogle Scholar
  • 19 Parveen A, Maqbool MT, Wang Y-H, Ali Z, Khan IA, Ashfaq MK. Evaluation of the hepatotoxic potential of Tinospora crispa and its isolated borapetosides B, C and F in a murine model. Planta Med 2020; 86 (07) 489-495
    Article in Thieme ConnectPubMedGoogle Scholar
  • 20 Hammad MA, Abdel-Bakky MS, Walker LA, Ashfaq MK. Tissue factor antisense deoxyoligonucleotide prevents monocrotaline/LPS hepatotoxicity in mice. J Appl Toxicol 2013; 33 (08) 774-783
    CrossrefPubMedGoogle Scholar
  • 21 Copple BL, Ganey PE, Roth RA. Liver inflammation during monocrotaline hepatotoxicity. Toxicology 2003; 190 (03) 155-169
    CrossrefPubMedGoogle Scholar
  • 22 Abdel-Bakky MSB, Hammad MA, Walkerit LA, Ashfaqi MK. Developing and characterizing a mouse model of hepatotoxicity using oral pyrrolizidine alkaloid (monocrotaline) administration, with potentiation of the liver injury by co-administration of LPS. Nat Prod Commun 2010; 5 (09) 1457-1462
    PubMedGoogle Scholar
  • 23 Bhakta T, Banerjee S, Mandal SC, Maity TK, Saha BP, Pal M. Hepatoprotective activity of Cassia fistula leaf extract. Phytomedicine 2001; 8 (03) 220-224
    CrossrefPubMedGoogle Scholar
  • 24 Gowda S, Desai PB, Hull VV, Math AAK, Vernekar SN, Kulkarni SS. A review on laboratory liver function tests. Pan Afr Med J 2009; 3 (17) 17
    PubMedGoogle Scholar
  • 25 Kennedy J. Herb and supplement use in the US adult population. Clin Ther 2005; 27 (11) 1847-1858
    CrossrefPubMedGoogle Scholar