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CC BY-NC-ND 4.0 · Journal of Coloproctology 2022; 42(03): 251-258
DOI: 10.1055/s-0042-1742668
Original Article

Serum Long-Noncoding RNA H19 and β-Catenin as Biomarkers for Early Diagnosis of Colorectal Cancer in Egyptian Patients: A Case Control Study

Ayat Abdelrahman Abdelrahman Elshazly 
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria, Egypt
,
Mohammed Nageeb Desouky
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria, Egypt
,
Iman Hassan Diab
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria, Egypt
,
Abeer Mahmoud Ali Ibrahim
2   Department of Internal Medicine, Faculty of Medicine, Alexandria University, Alexandria, Egypt
,
Fatma Ibrahim Dwedar
1   Department of Medical Biochemistry, Faculty of Medicine, Alexandria University, Alexandria, Egypt
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Abstract

Colorectal cancer (CRC) is the third most prevalent cancer and the second most common cause of cancer death; however, its early detection can improve the survival. Colonic polyps are considered one of the CRC's major risk factors. Throughout many biological processes and malignancies, the non-coding RNAs have essential functions. Certain long noncoding RNAs (lncRNAs), including H19, were supposed to be CRC possible biomarkers. Also, H19 has been reported to play a role in regulating the activity of β-catenin, a protein that regulates cell-to-cell adhesion, as well as gene transcription. The current work aimed to investigate the potential significance of LncRNA H19 relative serum expression level by quantitative polymerase chain reaction (q-PCR) and β-catenin by enzyme-linked immunosorbent assay (ELISA) as noninvasive biomarkers to discriminate between colorectal cancer and colonic polyps. The statistical analysis of the studied factors revealed that the serum expression of H19 and β-catenin in cancer cases were substantially greater than colonic polyp cases and normal control.

Conclusion The relative expressions of H19 and beta-catenin in the serum can significantly discriminate patients with CRC from those with polyp and normal controls, which could help when screening for CRC.

Keywords

LncRNA - H 19 - β-catenin - CRC - colonic polyp


Publication History

Received: 21 April 2021

Accepted: 06 August 2021

Article published online:
11 February 2022

© 2022. Sociedade Brasileira de Coloproctologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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