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CC BY 4.0 · Glob Med Genet 2022; 09(02): 129-140
DOI: 10.1055/s-0042-1743262
Original Article

Genetic Variants in Interleukin-10 Gene Association with Susceptibility and Cervical Cancer Development: A Case Control Study

Pushpendra D. Pratap
1   Central Research Laboratory, Molecular Diagnostic Unit, Department of Biochemistry, ERA's Lucknow Medical College, Era University, Lucknow, Uttar Pradesh, India
,
Syed Tasleem Raza
1   Central Research Laboratory, Molecular Diagnostic Unit, Department of Biochemistry, ERA's Lucknow Medical College, Era University, Lucknow, Uttar Pradesh, India
,
Ghazala Zaidi
2   Department of Allied Health Sciences, Era University, Lucknow, Uttar Pradesh, India
,
Shipra Kunwar
3   Department of Obstetrics & Gynecology, Era University, Lucknow, Uttar Pradesh, India
,
Sharique Ahmad
4   Department of Pathology, Era's Lucknow Medical College, Era University, Lucknow, Uttar Pradesh, India
,
Mark Rector Charles
1   Central Research Laboratory, Molecular Diagnostic Unit, Department of Biochemistry, ERA's Lucknow Medical College, Era University, Lucknow, Uttar Pradesh, India
,
Ale Eba
1   Central Research Laboratory, Molecular Diagnostic Unit, Department of Biochemistry, ERA's Lucknow Medical College, Era University, Lucknow, Uttar Pradesh, India
,
Muneshwar Rajput
1   Central Research Laboratory, Molecular Diagnostic Unit, Department of Biochemistry, ERA's Lucknow Medical College, Era University, Lucknow, Uttar Pradesh, India
› Author Affiliations Funding This study was financially supported by the Department of Biotechnology, Ministry of Science and Technology, New Delhi, Government of India (sanction number: 6242-P110/RGCB/PMD/DBT/SHKR/2015).
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Abstract

Objectives Cervical cancer (CC) is one of the most destructive disease caused by persistent HPV infection which affects women worldwide, especially in developing countries. The genetic basis of host immune response especially cytokine function has been shown to influence CC susceptibility. Studies have demonstrated that IL-10 gene polymorphism have been associated with numerous malignancies, but in context to CC results were inconclusive. Though, aim of our study to investigate the association between IL-10 -1082A/G and -819C/T promoter polymorphism and CC susceptibility.

Material and Methods This study comprised 192 women with CC and 200 controls. HPV detection was done by RT-PCR and genotyping was assessed through PCR-RFLP method. Serum concentration of IL-10 measured by ELISA.

Results Women with AG and AG+GG genotypes of IL-10 -1082A/G had two-fold increased risk of CC [OR, 2.35 (95% CI, 1.54–3.58), p = 0.005], [OR, 2.03 (95% CI, 1.36–3.04), p = 0.0005] compared to controls. Women with G allele of -1082A/G polymorphism had linked with CC susceptibility [OR, 1.39 (95% CI, 1.02–1.88), p = 0.036] compared to controls. No significant difference was found between patients and controls in the genotype or allele frequencies of IL–10 -819C/T polymorphism [OR, 1.00 (95% CI, 0.63–1.58), p = 0.99]. The level of serum concentration of IL-10 was significantly higher in cases compared to controls.

Conclusion These findings help to understand that polymorphism of IL-10 -1082A/G gene is associated with increased risk of CC development and can serve as a marker of genetic susceptibility to CC.

Keywords

human papillomavirus - cervical cancer - gene polymorphism


Publication History

Received: 27 November 2021

Accepted: 29 December 2021

Article published online:
25 February 2022

© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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