Abstract
Objectives This study aimed to determine some of bone molecular expressions and its possible
bone remodeling pathway between diabetes mellitus (DM) and osteoporosis model in the
mandibular bone of Wistar rats.
Materials and Methods Twenty-seven female Wistar rats were divided randomly into control and treatment
groups. Treatment groups were injected with streptozotocin intraperitoneally to induce
DM (P1) and underwent bilateral ovariectomy to generate osteoporosis (P2). All groups
were terminated after 12 weeks. Immunohistochemical and hematoxylin–eosin staining
were performed to determine the expression of Runt-related transcription factor 2
(RUNX2), Osterix, vascular endothelial growth factor (VEGF), receptor activator of
nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), tartrate-resistant acid phosphatase
(TRAP), and observed the osteoblast and osteoclast. Statistical analysis was performed
using one-way analysis of variance.
Results The lowest mean of RUNX2 and VEGF expression was found in the P2 group. The lowest
mean of Osterix expression was found in the P1 group. Both P1 and P2 groups of osteoblast/osteoclast
ratio were decreased. There were no significant differences in the expression of TRAP
between all groups; however, increased expression of RANKL/OPG ratio was only found
in the P2 group.
Conclusion DM and osteoporosis induce changes in the bone remodeling pathway which are represented
by a decrease in osteoblast biomarkers and an increase in osteoclast biomarkers.
Keywords
diabetes mellitus - osteoporosis - bone remodeling - medicine
