Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

Diatri Nari Ratih; Ema Mulyawati; Rika Kurnia Santi; Yulita Kristanti

doi:10.1055/s-0042-1746415

European Journal of Dentistry, Inhaltsverzeichnis

CC BY 4.0 · Eur J Dent 2023; 17(02): 398-402
DOI: 10.1055/s-0042-1746415

Original Article

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

Authors

Diatri Nari Ratih

¹Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
Ema Mulyawati

¹Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
Rika Kurnia Santi

²Former Student Specialist, Study Program of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia
Yulita Kristanti

¹Department of Conservative Dentistry, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia

Abstract

Objectives The aim of this study was to evaluate the addition of chitosan nanoparticle with concentrations of 0, 10, 20, and 30% to the epoxy resin-based (ERB) sealer on its antibacterial and cytotoxicity effect.

Methods and Materials This research was divided into two studies, the first study was the addition of chitosan with a concentration of 0% (as control), 10, 20, and 30% to an ERB sealer on its antibacterial effect, and the second study was on its cytotoxicity. An agar diffusion test was employed to determine the antibacterial effect on Enterococcus faecalis. An MTT (3-{4,5-dimethylthiazol-2-yl}-2,5-diphenyl tetrazolium bromide) assay was utilized to test the cytotoxicity by evaluating cell viability.

Statistical Analysis One-way analysis of variance and Tukey's test (α = 0.05) were used to analyze data obtained from each evaluation with a significance level of 95%.

Results The addition of chitosan nanoparticles at concentrations 10, 20, and 30% produced a greater inhibition zone of E. faecalis (p < 0.05), however, had less cytotoxicity compared with no addition of chitosan (0%) (p < 0.05).

Conclusion The addition of chitosan nanoparticles at concentrations 10, 20, and 30% to the ERB sealer produced greater antibacterial and less cytotoxicity compared with no addition of chitosan (0%).

Keywords

antibacterial - chitosan nanoparticle - cytotoxicity - root canal sealer

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Referenzen

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