Klin Padiatr 2022; 234(03): 183
DOI: 10.1055/s-0042-1748718
Abstracts

Diagnostic and functional role of extracellular vesicles (EV) from blood and CSF in pediatric medulloblastoma (MB)

L Reetz
1   Department of Pediatrics III, University Hospital Essen, Essen, Germany
,
J Ghanam
1   Department of Pediatrics III, University Hospital Essen, Essen, Germany
,
VK Chetty
1   Department of Pediatrics III, University Hospital Essen, Essen, Germany
,
D Reinhardt
1   Department of Pediatrics III, University Hospital Essen, Essen, Germany
,
BK Thakur
1   Department of Pediatrics III, University Hospital Essen, Essen, Germany
› Author Affiliations
 

Magnetic resonance imaging (MRI) and invasive surgery are being performed to examine the tumor tissue to detect and verify MB at diagnosis or relapse. Although some risk factors are established for a bad prognosis in MB, it remains unclear which MB patients will suffer from relapse after treatment. Thus, there is a great scope for the minimal invasive tool for detecting MB residual disease or relapse. Tumor-derived EVs were found to carry DNA, RNA, and proteins cargos with the same mutational status, suggesting their use in MB as an early biomarker. Therefore, we aimed to establish a liquid biopsy approach to characterize the EV-associated DNA-protein complex in MB patients’ blood and CSF. EVs were isolated using Size Exclusion Chromatography and Ultrafiltration from pediatric MB patients’ blood plasma and CSF. EVs were characterized using NTA, TEM, beads-based flow cytometry. Next, EV-DNA derived from blood and CSF of MB patients will be analyzed using the MB-NGS panel for early biomarker application. Functional EV-protein targets will be identified using LC-MS proteomics and EV interaction with the tumor microenvironment will be performed using cell and molecular biology tools.



Publication History

Article published online:
17 May 2022

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