The Diagnostic Value of Whole Blood lncRNA ENST00000550337.1 for Pre-Diabetes and Type 2 Diabetes Mellitus

Xuejie Li; Zhenzhou Zhao; Chuanyu Gao; Lixin Rao; Peiyuan Hao; Dongdong Jian; Wentao Li; Haiyu Tang; Muwei Li

doi:10.1055/s-0043-100018

Experimental and Clinical Endocrinology & Diabetes, Inhaltsverzeichnis

Exp Clin Endocrinol Diabetes 2017; 125(06): 377-383
DOI: 10.1055/s-0043-100018

Article

The Diagnostic Value of Whole Blood lncRNA ENST00000550337.1 for Pre-Diabetes and Type 2 Diabetes Mellitus

Autor*innen

Xuejie Li^*

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Zhenzhou Zhao^*

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Chuanyu Gao

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Lixin Rao

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Peiyuan Hao

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Dongdong Jian

²Department of Cardiology, The First Affiliated Hospital of Zhejiang University, Zhejiang University, Hangzhou, China.
Wentao Li

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Haiyu Tang

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Muwei Li

¹Department of Cardiology, People’s Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.

Abstract

Background

This study aims to investigate long noncoding RNA (lncRNA) as biomarker for pre-diabetes and T2DM.

Methods

LncRNAs in the peripheral blood of 6 healthy individuals and 6 T2DM patients were collected for microarray analysis. Then 5 candidate biomarkers from the differentially expressed lncRNAs were chosen and verified in a larger independent cohort (control group=20; pre-diabetes group=20; and T2DM group=20). The diagnostic capacity of ENST00000550337.1 was further tested in the third cohort (control group, n=60; pre-diabetes group, n=63; and T2DM group, n=64).

Results

A total of 17 lncRNAs were found to be differentially expressed between the 2 groups. 14 lncRNAs of these were upregulated in T2DM patients and 3 were downregulated. 5 upregulated lncRNAs were selected as potential biomarkers and verified in the second cohort, and the expression levels of 3 lncRNAs increased gradually from the control group to the pre-diabetes group to the T2DM group. The diagnostic value of ENST00000550337.1 was then tested in the third cohort, and its high diagnostic value for pre-diabetes and T2DM was confirmed.

Conclusions

LncRNA ENST00000550337.1 is a potential diagnostic biomarker for pre-diabetes and T2DM.

Key words

diabetes mellitus - type 2 - pre-diabetes - RNA - long noncoding - microarray analysis - biological markers

Volltext

Referenzen

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