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CC BY-NC-ND 4.0 · Planta Medica International Open 2017; 4(01): e24-e34
DOI: 10.1055/s-0043-105274
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Phytochemical Profiling and In Vitro Anticancer Activity of Purified Flavonoids of Andrographis glandulosa

Neeraja Cherukupalli
1   Centre for Plant Molecular Biology, Osmania University, Hyderabad, India
*   Contributed equally
,
Sudarshana Reddy Bhumireddy
2   National Centre for Mass Spectrometry, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
3   Academy of Scientific and Innovative Research, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
*   Contributed equally
,
Subrahmanya Sarma V Akella
4   Centre for NMR and Structural Chemistry, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad, India
,
Aaysha Sataniya
5   Chemical Biology Division, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad, India
,
Prabhakar Sripadi
2   National Centre for Mass Spectrometry, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
3   Academy of Scientific and Innovative Research, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana, India
*   Contributed equally
,
Venkateswara Rao Khareedu
1   Centre for Plant Molecular Biology, Osmania University, Hyderabad, India
,
Dashavantha Reddy Vudem
1   Centre for Plant Molecular Biology, Osmania University, Hyderabad, India
› Author Affiliations
Further Information

Publication History

received 15 November 2016
revised 18 February 2017

accepted 28 February 2017

Publication Date:
23 May 2017 (online)

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Abstract

The genus Andrographis includes diverse species of medicinal importance. The present study for the first time unravels the chemical profiling and medicinal importance of an unexplored species, Andrographis glandulosa. LC-MS analysis of A. glandulosa methanolic extract disclosed it as a rich source of flavonoids, with 8 minor peaks and 2 major peaks (m/z 285.0749 and 283.0595). NMR and ESI-MS/MS analyses confirmed the major peaks as (R) 2',5-dihydroxy-7-methoxyflavanone and 2',5-dihydroxy-7-methoxyflavone. Purified compounds exhibited antiproliferative activity on 3 cancer cell lines (HeLa, MIA PaCa and U-87) with IC50 39.81–46.21 µM for flavanone, and 20.84–25.16 µM for flavone. IC50 values of flavone are on a par with that of the positive control quercetin (21.78–26.82 µM). HeLa cells treated with 117 µM flavanone showed a loss of MMP (~55% of cells) resulting in apoptosis (~51% of cells). Treatment with 80 µM flavone caused a loss of MMP (~88% of cells) and induced apoptosis in ~70% of cells proving its efficacy over flavanone. Purified flavonoids exhibited cytotoxicity by disrupting mitochondrial membrane, caspase 3 activation and apoptosis. Purified flavonoids appear as promising natural products for developing cancer therapeutics.

Key words

Andrographis glandulosa - Acanthaceae - chemical profiling - LC-MS - NMR-spectroscopy - ESI-MS/MS - anticancer activity
 

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